ArticleProceedings of the National Academy of Sciences of the United States of America1986
Asymmetric lateral mobility of phospholipids in the human erythrocyte membrane.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 81 citations in OpenAlex.
- Erythrocytes membrane fluidity changes induced by adenylyl cyclase cascade activation: study using fluorescence recovery after photobleaching.European biophysics journal : EBJ · 2024Article
- Protein-free domains in native and ferroptosis-driven oxidized cell membranes: a molecular dynamics study of biophysical properties and doxorubicin uptake.Frontiers in molecular biosciences · 2024Article
- Lipidomic and Membrane Mechanical Signatures in Triple-Negative Breast Cancer: Scope for Membrane-Based Theranostics.Molecular and cellular biochemistry · 2022Review
- Structural and functional consequences of reversible lipid asymmetry in living membranes.Nature chemical biology · 2020Review
- Plasma membranes are asymmetric in lipid unsaturation, packing and protein shape.Nature chemical biology · 2020Article
- Plasma membrane asymmetry of lipid organization: fluorescence lifetime microscopy and correlation spectroscopy analysis.Journal of lipid research · 2020Article
- Dynamics of lipid domain formation: fluctuation analysis.Biochimica et biophysica acta · 2010Review
- Recent applications of fluorescence recovery after photobleaching (FRAP) to membrane bio-macromolecules.Sensors (Basel, Switzerland) · 2010Review
- Detection of lipid domains in model and cell membranes by fluorescence lifetime imaging microscopy of fluorescent lipid analogues.The Journal of biological chemistry · 2008Article
- Cholesterol depletion induces solid-like regions in the plasma membrane.Biophysical journal · 2006Article
- Ras diffusion is sensitive to plasma membrane viscosity.Biophysical journal · 2005Article
- Hydrogen-bonding propensities of sphingomyelin in solution and in a bilayer assembly: a molecular dynamics study.Biophysical journal · 2003Article
- Restricted accumulation of phosphatidylinositol 3-kinase products in a plasmalemmal subdomain during Fc gamma receptor-mediated phagocytosis.The Journal of cell biology · 2001Article
- Interactions between beta-enolase and creatine kinase in the cytosol of skeletal muscle cells.The Biochemical journal · 2000Article
- Translational diffusion of globular proteins in the cytoplasm of cultured muscle cells.Biophysical journal · 2000Article
- Interferometric fringe fluorescence photobleaching recovery interrogates entire cell surfaces.Biophysical journal · 1998Article
- Mobility of creatine phosphokinase and beta-enolase in cultured muscle cells.Biophysical journal · 1997Article
- Diffusion of fluorescently labeled macromolecules in cultured muscle cells.Biophysical journal · 1996Article
- Phospholipids in animal eukaryotic membranes: transverse asymmetry and movement.The Biochemical journal · 1993Review
- Phospholipid transmembrane domains and lateral diffusion in fibroblasts.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The fluorescent phospholipid 1-acyl-2-[12-(7-nitrobenz-2-oxa-1,3-diazol-4- yl)aminododecanoyl]phosphatidylcholine (NBD-phosphatidylcholine) and the corresponding aminophospholipid derivatives (NBD-phosphatidylethanolamine and NBD-phosphatidylserine) were introduced in the human erythrocyte membrane by a nonspecific phospholipid exchange protein purified from corn. The lateral mobility of the fluorescent phospholipids was measured by using an extension of the classical photobleaching recovery technique that takes advantage of a modulated fringe pattern and provides a high sensitivity. In intact erythrocytes and in ghosts resealed in the presence of ATP, the fluorescence-contrast curves after photobleaching decayed biexponentially corresponding to two lateral diffusion constants. With NBD-phosphatidylcholine, the majority of the signal corresponded to a "slow" component (1.08 X 10(-9) cm2/sec at 20 degrees C), whereas with the amino derivatives the majority of the signal corresponded to a "fast" component (5.14 X 10(-9) cm2/sec at 20 degrees C). If the ghosts were resealed without ATP, the fast component of the aminophospholipids disappeared. We interpret these results as follows: (i) Provided the cells or the ghosts contain ATP, the three fluorescent phospholipids distribute spontaneously between inner and outer leaflets as endogenous phospholipids, namely NBD-phosphatidylcholine is located in the outer leaflet, while both aminophospholipids are preferentially located in the inner leaflet. (ii) The viscosity of the inner leaflet of human erythrocyte membranes is lower than that of the outer leaflet.
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