Evidence map›Paper›PMID 34627253›Full record

ArticleMicrobial cell factories2021

Heterologous production of novel and rare C

Miho Takemura, Chiharu Takagi, Mayuri Aikawa, Kanaho Araki, Seon-Kang Choi, Mitsuhiro Itaya, Kazutoshi Shindo, Norihiko Misawa

Open access · goldAbstract read
In one paragraph

Article in Microbial cell factories, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.2field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 29 citations in OpenAlex.

  1. Article
  2. Characterization and Transcriptomic Analysis of AntarcticJournal of microbiology and biotechnology · 2026
    Article
  3. Article
  4. Characterization of CSynthetic and systems biotechnology · 2023
    Article
  5. Review
  6. Article
  7. Computational Insight into Intraspecies Distinctions inInternational journal of molecular sciences · 2023
    Article
  8. Antioxidant Potential and Capacity of Microorganism-Sourced CAntioxidants (Basel, Switzerland) · 2022
    Review
  9. Enhanced Production of CJournal of microbiology and biotechnology · 2022
    Article
  10. Review
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

Miho Takemura *Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University, 1-308 Suematsu, Nonoichi, Ishikawa, 921-8836, Japan. mtake@ishikawa-pu.ac.jp.ORCID http://orcid.org/0000-0001-5211-004X
Chiharu Takagi *Department of Food and Nutrition, Japan Women's University, Bunkyo-ku, Tokyo, 112-8681, Japan.
Mayuri AikawaDepartment of Food and Nutrition, Japan Women's University, Bunkyo-ku, Tokyo, 112-8681, Japan.
Kanaho ArakiDepartment of Food and Nutrition, Japan Women's University, Bunkyo-ku, Tokyo, 112-8681, Japan.
Seon-Kang ChoiDepartment of Agriculture and Life Industry, Kangwon National University, Chuncheon-si, Gangwon-do, 24341, Republic of Korea.
Mitsuhiro ItayaDepartment of Biomedical Engineering, Graduate School of Science and Technology, Shinshu University, Wakasato 4-17-1, Nagano, 380-8553, Japan.
Kazutoshi ShindoDepartment of Food and Nutrition, Japan Women's University, Bunkyo-ku, Tokyo, 112-8681, Japan.
Norihiko MisawaResearch Institute for Bioresources and Biotechnology, Ishikawa Prefectural University, 1-308 Suematsu, Nonoichi, Ishikawa, 921-8836, Japan.
Japan Women's University · JPIshikawa Prefectural University · JPKangwon National University · KRShinshu University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMembers of the genus Planococcus have been revealed to utilize and degrade solvents such as aromatic hydrocarbons and alkanes, and likely to acquire tolerance to solvents. A yellow marine bacterium Planococcus maritimus strain iso-3 was isolated from an intertidal sediment that looked industrially polluted, from the Clyde estuary in the UK. This bacterium was found to produce a yellow acyclic carotenoid with a basic carbon 30 (C

resultsA carotenoid biosynthesis gene cluster was found out through sequence analysis of the P. maritimus genomic DNA. This cluster consisted of seven carotenoid biosynthesis candidate genes (orf1-7). Then, we isolated the individual genes and analyzed the functions of these genes by expressing them in E. coli. The results indicated that orf2 and orf1 encoded 4,4'-diapophytoene synthase (CrtM) and 4,4'-diapophytoene desaturase (CrtNa), respectively. Furthermore, orf4 and orf5 were revealed to code for hydroxydiaponeurosporene desaturase (CrtNb) and glucosyltransferase (GT), respectively. By utilizing these carotenoid biosynthesis genes, we produced five intermediate C

conclusionsThe carotenoid biosynthesis genes from P. maritimus strain iso-3, were isolated and functionally identified. Furthermore, we were able to produce two novel and one rare C

Indexed as

PlanococcaceaeAntioxidantsCarotenoidsEscherichia coliGenes, BacterialAntioxidantsCarotenoids

Identifiers

PMID34627253
PMCPMC8502411
OpenAlexW3207768194

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.