Evidence map›Paper›PMID 34626412›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Application of a Mutant Cell Library to Determine the Structure-Function Relationship of Heparan Sulfate in Facilitating FGF2-FGFR1 Signaling.

John Faulkner, Xuehong Song, Lianchun Wang

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.7field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

John FaulknerDepartment of Molecular Pharmacology & Physiology, Morsani College of Medicine, University of South Florida, Tampa, FL, USA.
Xuehong SongDepartment of Molecular Pharmacology & Physiology, Morsani College of Medicine, University of South Florida, Tampa, FL, USA.
Lianchun WangDepartment of Molecular Pharmacology & Physiology, Morsani College of Medicine, University of South Florida, Tampa, FL, USA. lianchunw@usf.edu.
University of South Florida · US

Funding

TR&D3. Approaches to Proteoglycan Function at Biochemical and Cellular LevelsP41GM103390 · NIGMS · UNIVERSITY OF GEORGIA · PI MOREMEN, KELLEY W. · 2012 to 2019
$14.5M
Robo4 and heparan sulfate proteoglycan in angiogenesisR01HL093339 · NHLBI · UNIVERSITY OF SOUTH FLORIDA · PI WANG, LIANCHUN · 2009 to 2018
$3.4M
Heparan sulfate proteoglycan in the brain vascular clearance of amyloid-β and Alzheimer's diseaseRF1AG074289 · NIA · UNIVERSITY OF SOUTH FLORIDA · PI WANG, LIANCHUN · 2021 to 2021
$1.8M
Heparan sulfate proteoglycan in Alzheimer's diseaseR56AG062344 · NIA · UNIVERSITY OF SOUTH FLORIDA · PI WANG, LIANCHUN · 2018 to 2018
$785k
Using CRISPR-Cas9 technology to develop a mutant cell library for heparan sulfate structure-function studyR21HL131553 · NHLBI · UNIVERSITY OF GEORGIA · PI WANG, LIANCHUN · 2015 to 2016
$582k
NHLBI NIH HHS R01 HL093339NHLBI NIH HHS R21 HL131553NIA NIH HHS R56 AG062344NIA NIH HHS RF1 AG074289NIGMS NIH HHS P41 GM103390
6 · The paper itself

Abstract

Heparan sulfate (HS) is a linear polysaccharide with complex structures and modulates a wide range of biological functions. Elucidating the structure-function relationship of HS has been challenging. Recently, we generated a HS mutant mouse lung endothelial cell library by systematic deletion of HS genes expressed in the cell individually or in their combination. Here, we describe the experimental procedure using the mutant cell library to determine the structure-function relationship of HS in the regulation of FGF2-FGFR1 signaling at the levels of cell surface FGF2 binding and the downstream intracellular signaling activation. Our results demonstrated that strictly defined fine structure is required for HS to act as a co-receptor for FGF2-FGFR1 signaling.

Indexed as

Signal TransductionAnimalsCell MembraneFibroblast Growth Factor 2Heparan SulfateMiceReceptor, Fibroblast Growth Factor, Type 1Structure-Activity RelationshipFibroblast Growth Factor 2Heparan SulfateReceptor, Fibroblast Growth Factor, Type 1Cell signalingFGF2FGF2-FGFR1 signalingFGFR1Heparan sulfateMutant MLEC library

Identifiers

PMID34626412
OpenAlexW3203599775

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.