Evidence map›Paper›PMID 34626396›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Molecular Genetic Techniques for the Proteoglycan Functions in Drosophila.

Nanako Bowden, Masahiko Takemura, Hiroshi Nakato

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Nanako BowdenDepartment of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, USA.
Masahiko TakemuraDepartment of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, USA.
Hiroshi NakatoDepartment of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, MN, USA. nakat003@umn.edu.

Funding

Heparan sulfate proteoglycans in signaling and developmentR35GM131688 · NIGMS · UNIVERSITY OF MINNESOTA · PI Hiroshi Nakato · 2019 to 2026
$2.6M
NIGMS NIH HHS R35 GM131688
6 · The paper itself

Abstract

Several classes of heparan sulfate proteoglycan (HSPG) core proteins and all HS biosynthetic/modifying enzymes are evolutionarily conserved from human to Drosophila melanogaster. This genetically tractable model offers highly sophisticated techniques to manipulate gene function in a spatially and temporally controlled manner. Thus, Drosophila genetics has been a powerful system to explore functions of HSPGs in vivo. In this chapter, we will introduce three genetic techniques available in Drosophila: TARGET (temporal and regional gene expression targeting), MARCM (mosaic analysis with a repressible cell marker), and FLP-Out.

Indexed as

Drosophila melanogasterAnimalsGenetic TechniquesHeparan Sulfate ProteoglycansPhenotypeHeparan Sulfate ProteoglycansDrosophila melanogasterFLP-OutGAL4/UASHSPGMARCMTARGET

Identifiers

PMID34626396
PMCPMC8924912

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.