ArticleAngewandte Chemie (International ed. in English)2021
DNA Tiling Enables Precise Acylation-Based Labeling and Control of mRNA.
Article in Angewandte Chemie (International ed. in English), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- Nitro Reduction-Based RNA Control and Ultrafast Release.Angewandte Chemie (International ed. in English) · 2026Article
- Base modifications shift tertiary structure and activity in synthetic RNA origami and a natural ribozyme.Nature communications · 2026Article
- Sequence-Specific Installation of Aryl Groups in RNA via DNA-Catalyst Conjugates.Angewandte Chemie (International ed. in English) · 2025Article
- Localized 2'-OH Acylation at Poly(A) Extends RNA Translation.Journal of the American Chemical Society · 2025Article
- Engineering a DNA polymerase for modifying large RNA at specific positions.Nature chemistry · 2025Article
- Site-Selective Modification and Labeling of Native RNA.Chemistry (Weinheim an der Bergstrasse, Germany) · 2025Review
- Bioorthogonal masked acylating agents for proximity-dependent RNA labelling.Nature chemistry · 2024Article
- Direct Activation of Nucleobases with Small Molecules for the Conditional Control of Antisense Function.Angewandte Chemie (International ed. in English) · 2024Article
- 2'-OH as a universal handle for studying intracellular RNAs.Cell chemical biology · 2024Review
- Aqueous Activation of RNA 2'-OH for Conjugation with Amines and Thiols.Bioconjugate chemistry · 2024Article
- Efficient post-synthesis incorporation and conjugation of reactive ketones in RNAChemical communications (Cambridge, England) · 2023Article
- Stereoselective RNA reaction with chiral 2'-OH acylating agents.Chemical science · 2023Article
- Reversible 2'-OH acylation enhances RNA stability.Nature chemistry · 2023Article
- Diverse Reagent Scaffolds Provide Differential Selectivity of 2'-OH Acylation in RNA.Journal of the American Chemical Society · 2023Article
- Efficient DNA fluorescence labeling via base excision trapping.Nature communications · 2022Article
- Acylation probing of "generic" RNA libraries reveals critical influence of loop constraints on reactivity.Cell chemical biology · 2022Article
- Conjugation of RNAChemical communications (Cambridge, England) · 2022Article
- Nucleic Acids and Their Analogues for Biomedical Applications.Biosensors · 2022Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Methods for the site-selective labeling of long, native RNAs are needed for studying mRNA biology and future therapies. Current approaches involve engineering RNA sequences, which may alter folding, or are limited to specific sequences or bases. Here, we describe a versatile strategy for mRNA conjugation via a novel DNA-tiling approach. The method, TRAIL, exploits a pool of "protector" oligodeoxynucleotides to hybridize and block the mRNA, combined with an "inducer" DNA that extrudes a reactive RNA loop for acylation at a predetermined site. Using TRAIL, an azido-acylimidazole reagent was employed for labeling and controlling RNA for multiple applications in vitro and in cells, including analysis of RNA-binding proteins, imaging mRNA in cells, and analysis and control of translation. The TRAIL approach offers an efficient and accessible way to label and manipulate RNAs of virtually any length or origin without altering native sequence.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.