ArticleAngewandte Chemie (International ed. in English)2021
Real-Time Simultaneous Imaging of Acidification and Proteolysis in Single Phagosomes Using Bifunctional Janus-Particle Probes.
Article in Angewandte Chemie (International ed. in English), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 14 citations in OpenAlex.
- Bimodal expression of Type 3 Secretion System 2 enables cooperative virulence among intracellular Salmonella Typhimurium.PLoS pathogens · 2025Article
- Propulsive cell entry diverts pathogens from immune degradation by remodeling the phagocytic synapse.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Nanoparticles for Interrogation of Cell Signaling.Annual review of analytical chemistry (Palo Alto, Calif.) · 2023Review
- Propulsive cell entry diverts pathogens from immune degradation by remodeling the phagocytic synapse.bioRxiv : the preprint server for biology · 2023Article
- Bi-functional particles for real-time phagosome acidification and proteolysis multiplex assay in macrophages.Frontiers in immunology · 2023Article
- Probing cell membrane integrity using a histone-targeting protein nanocage displaying precisely positioned fluorophores.Nano research · 2023Article
- Domain-selective disruption and compression of phase-separated lipid vesicles by amphiphilic Janus nanoparticles.iScience · 2022Article
- A reversible plasmonic nanoprobe for dynamic imaging of intracellular pH during endocytosis.Chemical science · 2022Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The digestion of pathogens inside phagosomes by immune cells occurs through a sequence of reactions including acidification and proteolysis, but how the reactions are orchestrated in the right order is unclear due to a lack of methods to simultaneously measure more than one reaction in phagosomes. Here we report a bifunctional Janus-particle probe to simultaneously monitor acidification and proteolysis in single phagosomes in live cells. Each probe consists of a pH reporter and a proteolysis reporter that are spatially separated but function concurrently. Using the Janus probes, we found the acidic pH needed to initiate and maintain proteolysis, revealing the mechanism for the sequential occurrence of both reactions during pathogen digestion. We showed how bacterium-derived lipopolysaccharides alter the acidification and proteolysis in phagosomes. This study showcases Janus-particle probes as a generally applicable tool for monitoring multiple reactions in intracellular vesicles.
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Registered trials
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