ArticlePlant physiology2022
Riboswitch-mediated inducible expression of an astaxanthin biosynthetic operon in plastids.
Article in Plant physiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 35 citations in OpenAlex.
- Plastid Engineering for Photosynthesis-Driven Synthesis of Hyaluronic Acid in Tobacco.Plant biotechnology journal · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
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- RNA elements and their biotechnological applications in plants.The New phytologist · 2025Review
- The biology of grafting and its applications in studying information exchange between plants.Nature plants · 2025Review
- Multi-Omics Analysis Provides Insights into a Mosaic-Leaf Phenotype of Astaxanthin-Producing Tobacco.Plants (Basel, Switzerland) · 2025Article
- Regulation of nucleus-encoded trans-acting factors allows orthogonal fine-tuning of multiple transgenes in the chloroplast of Chlamydomonas reinhardtii.Plant biotechnology journal · 2025Article
- Recent trends and advances in chloroplast engineering and transformation methods.Frontiers in plant science · 2025Review
- A heat-inducible expression system for external control of gene expression in plastids.Plant biotechnology journal · 2024Article
- Plant Engineering to Enable Platforms for Sustainable Bioproduction of Terpenoids.Methods in molecular biology (Clifton, N.J.) · 2024Review
- An RNA thermometer in the chloroplast genome of Chlamydomonas facilitates temperature-controlled gene expression.Nucleic acids research · 2023Article
- Expression strategies for the efficient synthesis of antimicrobial peptides in plastids.Nature communications · 2022Article
- Review
- De-etiolation-induced protein 1 (DEIP1) mediates assembly of the cytochrome bNature communications · 2022Article
- Harnessing the Algal Chloroplast for Heterologous Protein Production.Microorganisms · 2022Review
- Going red but not mad: efficient astaxanthin production in tobacco without yield penalty.Plant physiology · 2022Article
- Transcriptional deregulation of stress-growth balance inFrontiers in plant science · 2022Article
- RNA structure mediated thermoregulation: What can we learn from plants?Frontiers in plant science · 2022Review
- Exploiting photosynthesis-driven P450 activity to produce indican in tobacco chloroplasts.Frontiers in plant science · 2022Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The high-value carotenoid astaxanthin (3,3'-dihydroxy-β,β-carotene-4,4'-dione) is one of the most potent antioxidants in nature. In addition to its large-scale use in fish farming, the pigment has applications as a food supplement and an active ingredient in cosmetics and in pharmaceuticals for the treatment of diseases linked to reactive oxygen species. The biochemical pathway for astaxanthin synthesis has been introduced into seed plants, which do not naturally synthesize this pigment, by nuclear and plastid engineering. The highest accumulation rates have been achieved in transplastomic plants, but massive production of astaxanthin has resulted in severe growth retardation. What limits astaxanthin accumulation levels and what causes the mutant phenotype is unknown. Here, we addressed these questions by making astaxanthin synthesis in tobacco (Nicotiana tabacum) plastids inducible by a synthetic riboswitch. We show that, already in the uninduced state, astaxanthin accumulates to similarly high levels as in transplastomic plants expressing the pathway constitutively. Importantly, the inducible plants displayed wild-type-like growth properties and riboswitch induction resulted in a further increase in astaxanthin accumulation. Our data suggest that the mutant phenotype associated with constitutive astaxanthin synthesis is due to massive metabolite turnover, and indicate that astaxanthin accumulation is limited by the sequestration capacity of the plastid.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.