ArticleiScience2021
Visualization and analysis of whole depot adipose tissue neural innervation.
Article in iScience, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed.
- Effects of 6-OHDA-Mediated Chemical Denervation of Sympathetic Axons in Inguinal Subcutaneous White Adipose Tissue.Diabetes, obesity & metabolism · 2026Article
- Targeting the peripheral nervous system in adipose tissue with engineered AAV vectors.Cell reports methods · 2026Article
- Adipose Tissue-Central Nervous System Axis in Obesity: Molecular Mechanisms, Inflammation, and Nutritional and Technological Implications.Metabolites · 2026Review
- Single cell transcriptomics of human weight loss links adipocyte NPY1R to control of lipolysis.Molecular metabolism · 2026Article
- Semaphorin 7A regulates axon outgrowth in subcutaneous white adipose tissue.Molecular metabolism · 2026Article
- Advances in Adipose Tissue Biology.Endocrine reviews · 2026Review
- Sensory-neuron-derived CGRPα controls white adipocyte differentiation and tissue plasticity.Cell reports · 2025Article
- An ultrasound-guided biopsy technique for obtaining supraclavicular brown fat biopsies and preadipocytes.Scientific reports · 2025Article
- Immersion-Based Clearing and Autofluorescence Quenching in Myocardial Tissue.Microcirculation (New York, N.Y. : 1994) · 2025Article
- Nanomedicine targeting PPAR in adipose tissue macrophages improves lipid metabolism and obesity-induced metabolic dysfunction.Science advances · 2025Article
- Clearing the path for whole-mount labeling and quantification of neuron and vessel density in adipose tissue.Journal of cell science · 2025Article
- Optimized immunofluorescence for liver structure analysis: Enhancing 3D resolution and minimizing tissue autofluorescence.Biology methods & protocols · 2025Review
- Cross-regulation between adipose tissue innervation and metaflammation: a potential therapeutic target for obesity.American journal of translational research · 2025Review
- Perivascular adipose tissue: a central player in the triad of diabetes, obesity, and cardiovascular health.Cardiovascular diabetology · 2024Review
- Interplay between the brain and adipose tissue: a metabolic conversation.EMBO reports · 2024Review
- Article
- Innervation of adipocytes is limited in mouse perivascular adipose tissue.American journal of physiology. Heart and circulatory physiology · 2024Article
- α2δ1-mediated maladaptive sensory plasticity disrupts adipose tissue homeostasis following spinal cord injury.Cell reports. Medicine · 2024Article
- Gene therapy approaches for obesity-induced adipose neuropathy: Device-targeted AAV-mediated neurotrophic factor delivery to adipocytes in subcutaneous adipose.Molecular therapy : the journal of the American Society of Gene Therapy · 2024Article
- Sensory nerve and neuropeptide diversity in adipose tissues.Molecules and cells · 2024Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
Abstract
Little is known about the diversity and function of adipose tissue nerves, due in part to the inability to effectively visualize the tissue's diverse nerve subtypes and the patterns of innervation across an intact depot. The tools to image and quantify adipose tissue innervation are currently limited. Here, we present a method of tissue processing that decreases tissue thickness in the z-axis while leaving cells intact for subsequent immunostaining. This was combined with autofluorescence quenching techniques to permit intact whole tissues to be mounted on slides and imaged by confocal microscopy, with a complementary means to perform whole tissue neurite density quantification after capture of tiled z-stack images. Additionally, we demonstrate how to visualize nerve terminals (the neuro-adipose nexus) in intact blocks of adipose tissue without z-depth reduction. We have included examples of data demonstrating nerve subtypes, neurovascular interactions, label-free imaging of collagen, and nerve bundle digital cross-sections.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.