Evidence map›Paper›PMID 34620908›Full record

ArticleScientific reports2021

Contamination-resistant, rapid emulsion-based isothermal nucleic acid amplification with Mie-scatter inspired light scatter analysis for bacterial identification.

Alexander S Day, Tiffany-Heather Ulep, Elizabeth Budiman, Laurel Dieckhaus, Babak Safavinia, Tyler Hertenstein, Jeong-Yeol Yoon

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Alexander S Day *Department of Biomedical Engineering, The University of Arizona, Tucson, AZ, 85721, USA.
Tiffany-Heather Ulep *Department of Biomedical Engineering, The University of Arizona, Tucson, AZ, 85721, USA.
Elizabeth BudimanDepartment of Biomedical Engineering, The University of Arizona, Tucson, AZ, 85721, USA.
Laurel DieckhausDepartment of Biomedical Engineering, The University of Arizona, Tucson, AZ, 85721, USA.
Babak SafaviniaDepartment of Biomedical Engineering, The University of Arizona, Tucson, AZ, 85721, USA.
Tyler HertensteinDepartment of Biomedical Engineering, The University of Arizona, Tucson, AZ, 85721, USA.
Jeong-Yeol YoonDepartment of Biomedical Engineering, The University of Arizona, Tucson, AZ, 85721, USA. jyyoon@arizona.edu.
University of Arizona · US

Funding

Cardiovascular Biomedical Engineering Training GrantT32HL007955 · NHLBI · UNIVERSITY OF ARIZONA · PI BARTON, JENNIFER KEHLET · 2001 to 2020
$5.1M
NHLBI NIH HHS T32 HL007955
6 · The paper itself

Abstract

An emulsion loop-mediated isothermal amplification (eLAMP) platform was developed to reduce the impact that contamination has on assay performance. Ongoing LAMP reactions within the emulsion droplets cause a decrease in interfacial tension, causing a decrease in droplet size, which results in decreased light scatter intensity due to Mie theory. Light scatter intensity was monitored via spectrophotometers and fiber optic cables placed at 30° and 60°. Light scatter intensities collected at 3 min, 30° were able to statistically differentiate 10

Indexed as

BacteriaDNA ContaminationDynamic Light ScatteringEmulsionsMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesPolymerase Chain ReactionSensitivity and SpecificityEmulsions

Identifiers

PMID34620908
PMCPMC8497611
OpenAlexW3202692618

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.