ArticleNature communications2021
A standardized genome architecture for bacterial synthetic biology (SEGA).
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- A portable Cas6f-based system for multiplex translational repression in bacteria.Nature communications · 2026Article
- Wrinkle-like structures emerge from matrix complementarity and mechanical discontinuity in heterogenous biofilms.NPJ biofilms and microbiomes · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- tRNA-Mediated Plasmid Stabilization for Antibiotic-Free Applications inACS synthetic biology · 2026Article
- Simple and robust in vivo engineering of plasmid DNA at any copy number in Escherichia coli.Communications biology · 2025Article
- Modular plasmid design for autonomous multi-protein expression in Escherichia coli.Journal of biological engineering · 2025Article
- Engineering is evolution: a perspective on design processes to engineer biology.Nature communications · 2024Article
- SEVA 4.0: an update of the Standard European Vector Architecture database for advanced analysis and programming of bacterial phenotypes.Nucleic acids research · 2023Article
- Efficient Bacterial Genome Engineering throughout the Central Dogma Using the Dual-Selection MarkerACS synthetic biology · 2022Article
- Genetic context effects can override canonical cis regulatory elements in Escherichia coli.Nucleic acids research · 2022Article
- Glucose-Derived Raspberry Ketone Produced via EngineeredFrontiers in bioengineering and biotechnology · 2022Article
- DART takes aim at community editing.Nature microbiology · 2022Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chromosomal recombinant gene expression offers a number of advantages over plasmid-based synthetic biology. However, the methods applied for bacterial genome engineering are still challenging and far from being standardized. Here, in an attempt to realize the simplest recombinant genome technology imaginable and facilitate the transition from recombinant plasmids to genomes, we create a simplistic methodology and a comprehensive strain collection called the Standardized Genome Architecture (SEGA). In its simplest form, SEGA enables genome engineering by combining only two reagents: a DNA fragment that can be ordered from a commercial vendor and a stock solution of bacterial cells followed by incubation on agar plates. Recombinant genomes are identified by visual inspection using green-white colony screening akin to classical blue-white screening for recombinant plasmids. The modular nature of SEGA allows precise multi-level control of transcriptional, translational, and post-translational regulation. The SEGA architecture simultaneously supports increased standardization of genetic designs and a broad application range by utilizing well-characterized parts optimized for robust performance in the context of the bacterial genome. Ultimately, its adaption and expansion by the scientific community should improve predictability and comparability of experimental outcomes across different laboratories.
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