Evidence map›Paper›PMID 34619353›Full record

ArticleMitochondrion2021

Remdesivir triphosphate blocks DNA synthesis and increases exonucleolysis by the replicative mitochondrial DNA polymerase, Pol γ.

Elena J Ciesielska, Shalom Kim, Hyacintha-Ghislaine M Bisimwa, Cody Grier, Md Mostafijur Rahman, Carolyn K J Young, Matthew J Young, Marcos T Oliveira, Grzegorz L Ciesielski

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Mitochondrion, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Elena J CiesielskaDepartment of Chemistry, Auburn University at Montgomery, Montgomery, AL 36117, United States.
Shalom KimDepartment of Chemistry, Auburn University at Montgomery, Montgomery, AL 36117, United States.
Hyacintha-Ghislaine M BisimwaDepartment of Chemistry, Auburn University at Montgomery, Montgomery, AL 36117, United States.
Cody GrierDepartment of Chemistry, Auburn University at Montgomery, Montgomery, AL 36117, United States.
Md Mostafijur RahmanDepartment of Biochemistry and Molecular Biology, Southern Illinois University School of Medicine, Carbondale, IL 62901, United States.
Carolyn K J YoungDepartment of Biochemistry and Molecular Biology, Southern Illinois University School of Medicine, Carbondale, IL 62901, United States.
Matthew J YoungDepartment of Biochemistry and Molecular Biology, Southern Illinois University School of Medicine, Carbondale, IL 62901, United States.
Marcos T OliveiraDepartamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista "Júlio de Mesquita Filho", Jaboticabal, SP, Brazil.
Grzegorz L CiesielskiDepartment of Chemistry, Auburn University at Montgomery, Montgomery, AL 36117, United States. Electronic address: gciesiel@aum.edu.
Auburn University at Montgomery · USSouthern Illinois University School of Medicine · USUniversidade Estadual Paulista (Unesp) · BR

Funding

Visual Phenotyping CoreP30EY003039 · NEI · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI Paul Douglas Gamlin · 1985 to 2026
$16.9M
The Mechanism of Elimination of the Mitochondrial DNA ReplisomeR15GM139104 · NIGMS · UNIVERSITY OF NORTH FLORIDA · PI Grzegorz Leszek Ciesielski · 2021 to 2026
$1.1M
The Roles of Polymerase Gamma Accessory Subunit Gene Mutations in Human Disease.R00ES022638 · NIEHS · SOUTHERN ILLINOIS UNIVERSITY CARBONDALE · PI YOUNG, MATTHEW J · 2016 to 2018
$747k
NEI NIH HHS P30 EY003039NIEHS NIH HHS R00 ES022638NIGMS NIH HHS R15 GM139104
6 · The paper itself

Abstract

The COVID-19 pandemic prompted the FDA to authorize a new nucleoside analogue, remdesivir, for emergency use in affected individuals. We examined the effects of its active metabolite, remdesivir triphosphate (RTP), on the activity of the replicative mitochondrial DNA polymerase, Pol γ. We found that while RTP is not incorporated by Pol γ into a nascent DNA strand, it remains associated with the enzyme impeding its synthetic activity and stimulating exonucleolysis. In spite of that, we found no evidence for deleterious effects of remdesivir treatment on the integrity of the mitochondrial genome in human cells in culture.

Indexed as

COVID-19 Drug TreatmentSARS-CoV-2Adenosine MonophosphateAlanineCells, CulturedCOVID-19DNA, MitochondrialDNA Polymerase gammaDNA ReplicationFibroblastsHumansAdenosine MonophosphateAlanineDNA, MitochondrialDNA Polymerase gammaPOLG protein, humanremdesivirAntiviral nucleoside analoguesCOVID-19DNA polymerase gammaMitochondrial DNARemdesivir

Identifiers

PMID34619353
PMCPMC8595818
OpenAlexW3204540146

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.