ArticleScience (New York, N.Y.)2021
Reconstitution of an intact clock reveals mechanisms of circadian timekeeping.
Article in Science (New York, N.Y.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed.
- From phenomenon to mechanism in the circadian clock of Synechococcus elongatus.Npj biological timing and sleep · 2026Review
- Circadian temperature compensation is intrinsically linked with metabolism and redox signaling.Nature communications · 2026Article
- Cyanobacterial Circadian Clock: Molecular Mechanisms and Physiological Outputs.Plants (Basel, Switzerland) · 2026Review
- An underlying bistability sets amplitude and explains temperature compensation in the cyanobacterial circadian clock.bioRxiv : the preprint server for biology · 2026Article
- Revitalizing the "biology" in "chronobiology".Npj biological timing and sleep · 2026Review
- The Phenotypic Landscape of a Circadian Clock.bioRxiv : the preprint server for biology · 2026Article
- Intrinsic period stability of the cyanobacterial circadian oscillator across in vitro and in vivo conditions.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Effects of the signaling molecule cyclic-di-GMP on cyanobacterial circadian rhythm inJournal of bacteriology · 2026Article
- Effects of sodium chloride on circadian period and temperature compensation of KaiC phosphorylation.Scientific reports · 2026Article
- Mechanism and reconstitution of circadian transcription in cyanobacteria.Nature structural & molecular biology · 2026Article
- Clock-Dependent Phosphorylation of CikA Regulates Its Activity.Journal of biological rhythms · 2025Article
- A novel circadian behavior rhythm in arrhythmiciScience · 2025Article
- Mutations in the circadian cycle drive adaptive plasticity in cyanobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Reconstitution of circadian clock in synthetic cells reveals principles of timekeeping.Nature communications · 2025Article
- Temporal regulation of metabolic processes in the marine diazotrophbioRxiv : the preprint server for biology · 2025Article
- Article
- Evolutionary origins of self-sustained Kai protein circadian oscillators in cyanobacteria.Nature communications · 2025Article
- The priming phosphorylation of KaiC is activated by the release of its autokinase autoinhibition.PNAS nexus · 2025Article
- Life on Venus?Life (Basel, Switzerland) · 2025Article
- When lowering temperature, the in vivo circadian clock in cyanobacteria follows and surpasses the in vitro protein clock trough the Hopf bifurcation.Scientific reports · 2025Article
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14 authors.
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Abstract
Circadian clocks control gene expression to provide an internal representation of local time. We report reconstitution of a complete cyanobacterial circadian clock in vitro, including the central oscillator, signal transduction pathways, downstream transcription factor, and promoter DNA. The entire system oscillates autonomously and remains phase coherent for many days with a fluorescence-based readout that enables real-time observation of each component simultaneously without user intervention. We identified the molecular basis for loss of cycling in an arrhythmic mutant and explored fundamental mechanisms of timekeeping in the cyanobacterial clock. We find that SasA, a circadian sensor histidine kinase associated with clock output, engages directly with KaiB on the KaiC hexamer to regulate period and amplitude of the central oscillator. SasA uses structural mimicry to cooperatively recruit the rare, fold-switched conformation of KaiB to the KaiC hexamer to form the nighttime repressive complex and enhance rhythmicity of the oscillator, particularly under limiting concentrations of KaiB. Thus, the expanded in vitro clock reveals previously unknown mechanisms by which the circadian system of cyanobacteria maintains the pace and rhythmicity under variable protein concentrations.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.