Evidence map›Paper›PMID 34618430›Full record

ArticleACS nano2021

Direct Nanopore Sequencing of Individual Full Length tRNA Strands.

Niki K Thomas, Vinay C Poodari, Miten Jain, Hugh E Olsen, Mark Akeson, Robin L Abu-Shumays

Open access · greenAbstract read
In one paragraph

Article in ACS nano, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.

0numbers the graph read from it
0cells of the map it votes in
80citing papers in PubMed
6.6field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

80 citing papers in PubMed, 123 citations in OpenAlex.

  1. Article
  2. Article
  3. tRNA modifications in viral replication.The Journal of biological chemistry · 2026
    Review
  4. Review
  5. RNase 4 improves bottom-up modification mapping ofbioRxiv : the preprint server for biology · 2026
    Article
  6. Article
  7. Pseudouridylation landscape across 42bioRxiv : the preprint server for biology · 2026
    Article
  8. Article
  9. Review
  10. Review
  11. Review
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Review
  18. Article
  19. Article
  20. Article

20 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Niki K ThomasBiomolecular Engineering Department, Genomics Institute, and Center for Molecular Biology of RNA University of California, Santa Cruz, California 95064, United States.ORCID 0000-0003-2642-2570
Vinay C PoodariBiomolecular Engineering Department, Genomics Institute, and Center for Molecular Biology of RNA University of California, Santa Cruz, California 95064, United States.
Miten JainBiomolecular Engineering Department, Genomics Institute, and Center for Molecular Biology of RNA University of California, Santa Cruz, California 95064, United States.ORCID 0000-0002-4571-3982
Hugh E OlsenBiomolecular Engineering Department, Genomics Institute, and Center for Molecular Biology of RNA University of California, Santa Cruz, California 95064, United States.
Mark AkesonBiomolecular Engineering Department, Genomics Institute, and Center for Molecular Biology of RNA University of California, Santa Cruz, California 95064, United States.
Robin L Abu-ShumaysBiomolecular Engineering Department, Genomics Institute, and Center for Molecular Biology of RNA University of California, Santa Cruz, California 95064, United States.ORCID 0000-0003-1485-080X
University of California, Santa Cruz · US

Funding

A Unified Nanopore Platform for Direct Sequencing of Individual Full Length RNA Strands Bearing Modified NucleotidesR01HG010053 · NHGRI · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI AKESON, MARK A · 2018 to 2021
$3.8M
UCSC IMSDT32GM135742 · NIGMS · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI Melissa S Jurica · 2020 to 2026
$2.7M
NHGRI NIH HHS R01 HG010053NIGMS NIH HHS T32 GM135742
6 · The paper itself

Abstract

We describe a method for direct tRNA sequencing using the Oxford Nanopore MinION. The principal technical advance is custom adapters that facilitate end-to-end sequencing of individual transfer RNA (tRNA) molecules at subnanometer precision. A second advance is a nanopore sequencing pipeline optimized for tRNA. We tested this method using purified

Indexed as

NanoporesNanopore SequencingEscherichia coliHigh-Throughput Nucleotide SequencingHumansNucleotidesNucleotidesE. coliisoacceptorisodecodermodificationsnanoporepseudouridinetRNA

Identifiers

PMID34618430
PMCPMC10189790
OpenAlexW3202467862

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.