ArticlePlant physiology2021
Cytotoxic function of xylanase VdXyn4 in the plant vascular wilt pathogen Verticillium dahliae.
Article in Plant physiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 55 citations in OpenAlex.
- Article
- The deletion of a major facilitator superfamily geneMicrobiology spectrum · 2026Article
- Plant-associated fungi co-opt ancient antimicrobials for host manipulation.Science advances · 2026Article
- Cloning, heterologous expression, and characterization of a metagenome-derived GH10 xylanase with salt and alkali tolerance from Xinjiang saline-alkali soil.Antonie van Leeuwenhoek · 2026Article
- VdEGe1, a cell wall-degrading enzyme gene from Verticillium dahliae required for cotton pathogenesis.BMC microbiology · 2026Article
- Dual-Role GH7 Family Proteins From Verticillium dahliae Function as Virulence Factors and Intracellular Effectors Triggering NLR-Mediated Immunity.Plant, cell & environment · 2026Article
- Verticillium dahliae Secretory Aspartyl Protease VdSAP Targets Cotton GhARP to Modulate Plant Defence and Defoliation.Molecular plant pathology · 2025Article
- Recognition of a Fungal Effector Potentiates Pathogen-Associated Molecular Pattern-Triggered Immunity in Cotton.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Dual Transcriptome Analysis Reveals the Changes in Gene Expression in Both Cotton andJournal of fungi (Basel, Switzerland) · 2024Article
- Insights into the biocontrol and plant growth promotion functions of Bacillus altitudinis strain KRS010 against Verticillium dahliae.BMC biology · 2024Article
- A Glycosyl Hydrolase 5 Family Protein Is Essential for Virulence of Necrotrophic Fungi and Can Suppress Plant Immunity.International journal of molecular sciences · 2024Article
- The elicitor VP2 from Verticillium dahliae triggers defence response in cotton.Plant biotechnology journal · 2024Article
- Article
- Molecular warfare between pathogenic Fusarium oxysporum R1 and host Crocus sativus L. unraveled by dual transcriptomics.Plant cell reports · 2024Article
- Global characterization of GH11 family xylanases genes inFrontiers in microbiology · 2024Article
- Article
- Polyethyleneimine-coated MXene quantum dots improve cotton tolerance to Verticillium dahliae by maintaining ROS homeostasis.Nature communications · 2023Article
- Article
- Article
- VdERG2 was involved in ergosterol biosynthesis, nutritional differentiation and virulence of Verticillium dahliae.Current genetics · 2023Article
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phytopathogen xylanases play critical roles in pathogenesis, likely due to their ability to degrade plant structural barriers and manipulate host immunity. As an invader of plant xylem vessels, the fungus Verticillium dahliae is thought to deploy complex cell wall degrading enzymes. Comparative genomics analyses revealed that the V. dahliae genome encodes a family of six xylanases, each possessing a glycosyl hydrolase 11 domain, but the functions of these enzymes are undetermined. Characterizing gene deletion mutants revealed that only V. dahliae xylanase 4 (VdXyn4) degraded the plant cell wall and contributed to the virulence of V. dahliae. VdXyn4 displayed cytotoxic activity and induced a necrosis phenotype during the late stages of infection, leading to vein and petiole collapse that depended on the enzyme simultaneously localizing to nuclei and chloroplasts. The internalization of VdXyn4 was in conjunction with that of the plasma membrane complexLeucine-rich repeat (LRR)-receptor-like kinase suppressor of BIR1-1 (SOBIR1)/LRR-RLK BRI1-associated kinase-1 (BAK1), but we could not rule out the possibility that VdXyn4 may also act as an apoplastic effector. Immune signaling (in the SA-JA pathways) induced by VdXyn4 relative to that induced by known immunity effectors was substantially delayed. While cytotoxic activity could be partially suppressed by known effectors, they failed to impede necrosis in Nicotiana benthamiana. Thus, unlike typical effectors, cytotoxicity of VdXyn4 plays a crucial intracellular role at the late stages of V. dahliae infection and colonization, especially following pathogen entry into the xylem; this cytotoxic activity is likely conserved in the corresponding enzyme families in plant vascular pathogens.
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