ArticlePlant physiology2021
DIOXYGENASE FOR AUXIN OXIDATION 1 catalyzes the oxidation of IAA amino acid conjugates.
Article in Plant physiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 46 citations in OpenAlex.
- Metabolism meets transport: a new perspective on auxin homeostasis at subcellular level.Planta · 2026Review
- GH3 phylogenetic subgroups define divergent routes of auxin inactivation via aspartate and glutamine conjugation.Plant physiology · 2026Article
- Review
- Comprehensive characterisation of IAA inactivation pathways reveals the impact of glycosylation on auxin metabolism and plant development in Arabidopsis.Communications biology · 2026Article
- Tobacco two-pore calcium channel 1a is localised at the tonoplast, but acts on events at the plasma membrane.Protoplasma · 2026Article
- Membrane transport of root-borne trans-zeatin riboside maintains the cytokinin homeostasis in shoots.Journal of experimental botany · 2025Article
- Mechanisms of auxin action in plant growth and development.Nature reviews. Molecular cell biology · 2025Review
- Phenylacetic acid metabolism in land plants: novel pathways and metabolites.Journal of experimental botany · 2025Article
- Cytosolic- and membrane-localized oxidized indole-3-acetic acid formation regulates developmental auxin transients.Plant physiology · 2025Article
- Chitosan stimulates root hair callose deposition, endomembrane dynamics, and inhibits root hair growth.Plant, cell & environment · 2025Article
- An auxin research odyssey: 1989-2023.The Plant cell · 2024Review
- Multiple Physiological and Biochemical Functions of Ascorbic Acid in Plant Growth, Development, and Abiotic Stress Response.International journal of molecular sciences · 2024Review
- RNA Sequencing Analysis and Verification ofPlants (Basel, Switzerland) · 2024Article
- Occurrence, Function, and Biosynthesis of the Natural Auxin Phenylacetic Acid (PAA) in Plants.Plants (Basel, Switzerland) · 2023Review
- Metabolic profiles of 2-oxindole-3-acetyl-amino acid conjugates differ in various plant species.Frontiers in plant science · 2023Article
- High-throughput interspecies profiling of acidic plant hormones using miniaturised sample processing.Plant methods · 2022Article
- Chemical inhibition of the auxin inactivation pathway uncovers the roles of metabolic turnover in auxin homeostasis.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Inactivation of the entire Arabidopsis group II GH3s confers tolerance to salinity and water deficit.The New phytologist · 2022Article
- Article
- The main oxidative inactivation pathway of the plant hormone auxin.Nature communications · 2021Article
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Authors and funding
15 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Together with auxin transport, auxin metabolism is a key determinant of auxin signaling output by plant cells. Enzymatic machinery involved in auxin metabolism is subject to regulation based on numerous inputs, including the concentration of auxin itself. Therefore, experiments characterizing altered auxin availability and subsequent changes in auxin metabolism could elucidate the function and regulatory role of individual elements in the auxin metabolic machinery. Here, we studied auxin metabolism in auxin-dependent tobacco BY-2 cells. We revealed that the concentration of N-(2-oxindole-3-acetyl)-l-aspartic acid (oxIAA-Asp), the most abundant auxin metabolite produced in the control culture, dramatically decreased in auxin-starved BY-2 cells. Analysis of the transcriptome and proteome in auxin-starved cells uncovered significant downregulation of all tobacco (Nicotiana tabacum) homologs of Arabidopsis (Arabidopsis thaliana) DIOXYGENASE FOR AUXIN OXIDATION 1 (DAO1), at both transcript and protein levels. Auxin metabolism profiling in BY-2 mutants carrying either siRNA-silenced or CRISPR-Cas9-mutated NtDAO1, as well as in dao1-1 Arabidopsis plants, showed not only the expected lower levels of oxIAA, but also significantly lower abundance of oxIAA-Asp. Finally, ability of DAO1 to oxidize IAA-Asp was confirmed by an enzyme assay in AtDAO1-producing bacterial culture. Our results thus represent direct evidence of DAO1 activity on IAA amino acid conjugates.
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