ArticlePlant physiology2021
LATERAL ORGAN BOUNDARIES DOMAIN 25 functions as a key regulator of haustorium development in dodders.
Article in Plant physiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 34 citations in OpenAlex.
- Graft compatibility emerges from the balance between regeneration and immunity: lessons from plant-plant interactions.Frontiers in plant science · 2026Review
- Current understanding of the role of the cell wall in Cuscuta parasitism.Plant biology (Stuttgart, Germany) · 2025Review
- A unique phytochrome B gene in Cuscuta campestris and its responses during the initial stage of haustorium formation.Journal of plant research · 2025Article
- From spore to gametophyte: Investigating morphological dynamics of hyaline cells and corresponding gene expression patterns in Sphagnum.BMC plant biology · 2025Article
- One organ to infect them all: the Cuscuta haustorium.Annals of botany · 2025Review
- Involvement of MID1-COMPLEMENTING ACTIVITY 1 encoding a mechanosensitive ion channel in prehaustorium development of the stem parasitic plant Cuscuta campestris.Plant & cell physiology · 2025Article
- Molecular Mechanism ofMetabolites · 2025Article
- Large-scale interplant exchange of macromolecules between soybean and dodder under nutrient stresses.Plant diversity · 2024Article
- Pectin modifications promote haustoria development in the parasitic plant Phtheirospermum japonicum.Plant physiology · 2023Article
- Trans-species microRNA loci in the parasitic plant Cuscuta campestris have a U6-like snRNA promoter.The Plant cell · 2023Article
- The plant vampire diaries: a historic perspective on Cuscuta research.Journal of experimental botany · 2023Review
- Genomic and Epigenomic Mechanisms of the Interaction between Parasitic and Host Plants.International journal of molecular sciences · 2023Review
- Protein Profiling ofPlants (Basel, Switzerland) · 2023Article
- CRISPR gene editing to improve crop resistance to parasitic plants.Frontiers in genome editing · 2023Review
- A host-free transcriptome for haustoriogenesis in Cuscuta campestris: Signature gene expression identifies markers of successive development stages.Physiologia plantarum · 2022Article
- Gene regulatory networks for compatible versus incompatible grafts identify a role for SlWOX4 during junction formation.The Plant cell · 2022Article
- Review
- Regulatory Modules Involved in the Degradation and Modification of Host Cell Walls DuringFrontiers in plant science · 2022Article
- Transcriptional Basis for Haustorium Formation and Host Establishment in HemiparasiticFrontiers in genetics · 2022Article
- Transcription factor PagLBD21 functions as a repressor of secondary xylem development inForestry research · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Parasitic plants reduce crop yield worldwide. Dodder (Cuscuta campestris) is a stem parasite that attaches to its host, using haustoria to extract nutrients and water. We analyzed the transcriptome of six C. campestris tissues and identified a key gene, LATERAL ORGAN BOUNDARIES DOMAIN 25 (CcLBD25), as highly expressed in prehaustoria and haustoria. Gene coexpression networks from different tissue types and laser-capture microdissection RNA-sequencing data indicated that CcLBD25 could be essential for regulating cell wall loosening and organogenesis. We employed host-induced gene silencing by generating transgenic tomato (Solanum lycopersicum) hosts that express hairpin RNAs to target and down-regulate CcLBD25 in the parasite. Our results showed that C. campestris growing on CcLBD25 RNAi transgenic tomatoes transited to the flowering stage earlier and had reduced biomass compared with C. campestris growing on wild-type (WT) hosts, suggesting that parasites growing on transgenic plants were stressed due to insufficient nutrient acquisition. We developed an in vitro haustorium system to assay the number of prehaustoria produced on strands from C. campestris. Cuscuta campestris grown on CcLBD25 RNAi tomatoes produced fewer prehaustoria than those grown on WT tomatoes, indicating that down-regulating CcLBD25 may affect haustorium initiation. Cuscuta campestris haustoria growing on CcLBD25 RNAi tomatoes exhibited reduced pectin digestion and lacked searching hyphae, which interfered with haustorium penetration and formation of vascular connections. The results of this study elucidate the role of CcLBD25 in haustorium development and might contribute to developing parasite-resistant crops.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.