ArticlePlant physiology2021
The histone variant H3.3 promotes the active chromatin state to repress flowering in Arabidopsis.
Article in Plant physiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 39 citations in OpenAlex.
- A chromosome-level genome assembly of Vanilla planifolia uncovers the genomic architecture underlying partial endoreplication.BMC genomics · 2026Article
- Histone variant H2A.X represses deposition of active H3K4me3 marks and restricts H2A.W incorporation.Nucleic acids research · 2026Article
- Epigenetic regulation of floral transition: pathways and players.Plant cell reports · 2026Review
- Improved Longan Genome Assembly Reveals Insights Into Flowering Mechanisms.Plant biotechnology journal · 2026Article
- The genomic basis of local adaptation to photoperiod across altitude in a self-fertilizing monkeyflower.bioRxiv : the preprint server for biology · 2026Article
- PRC1-mediated H2A.Zub promotes gene expression by preventing H3.1K27me1 incorporation in Arabidopsis.Genome biology · 2025Article
- DDM1 Maintains Heterochromatin by Regulating Histone Variants.International journal of molecular sciences · 2025Review
- Single amino acid mutations in histone H3.3 illuminate the functional significance of H3K4 methylation in plants.Nature communications · 2025Article
- H2A.Z removal mediates the activation of genes accounting for cell elongation under light and temperature stress.Plant cell reports · 2024Review
- Revealing cis- and trans-regulatory elements underlying nuclear distribution and function of the Arabidopsis histone H2B.8 variant.BMC plant biology · 2024Article
- Mind the gap: Epigenetic regulation of chromatin accessibility in plants.Plant physiology · 2024Review
- The Histone Variant H3.3 Is Required for Plant Growth and Fertility inInternational journal of molecular sciences · 2024Article
- Do plant histone variants stand idly by while DNA viruses invade the nucleus?Stress biology · 2023Article
- PIF7-mediated epigenetic reprogramming promotes the transcriptional response to shade in Arabidopsis.The EMBO journal · 2023Article
- Advances in biological functions and mechanisms of histone variants in plants.Frontiers in genetics · 2023Review
- Histone H3.3 deposition in seed is essential for the post-embryonic developmental competence in Arabidopsis.Nature communications · 2022Article
- Mutation of histone H3 serine 28 to alanine influences H3K27me3-mediated gene silencing in Arabidopsis thaliana.Plant physiology · 2022Article
- Histone Variants in the Specialization of Plant Chromatin.Annual review of plant biology · 2022Review
- Recent advances in the chromatin-based mechanism ofFrontiers in plant science · 2022Review
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The histone H3 family in animals and plants includes replicative H3 and nonreplicative H3.3 variants. H3.3 preferentially associates with active transcription, yet its function in development and transcription regulation remains elusive. The floral transition in Arabidopsis (Arabidopsis thaliana) involves complex chromatin regulation at a central flowering repressor FLOWERING LOCUS C (FLC). Here, we show that H3.3 upregulates FLC expression and promotes active histone modifications histone H3 lysine 4 trimethylation (H3K4me3) and histone H3 lysine 36 trimethylation (H3K36me3) at the FLC locus. The FLC activator FRIGIDA (FRI) directly mediates H3.3 enrichment at FLC, leading to chromatin conformation changes and further induction of active histone modifications at FLC. Moreover, the antagonistic H3.3 and H2A.Z act in concert to activate FLC expression, likely by forming unstable nucleosomes ideal for transcription processing. We also show that H3.3 knockdown leads to H3K4me3 reduction at a subset of particularly short genes, suggesting the general role of H3.3 in promoting H3K4me3. The finding that H3.3 stably accumulates at FLC in the absence of H3K36me3 indicates that the H3.3 deposition may serve as a prerequisite for active histone modifications. Our results reveal the important function of H3.3 in mediating the active chromatin state for flowering repression.
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