ArticlePlant physiology2022
X-ray microscopy enables multiscale high-resolution 3D imaging of plant cells, tissues, and organs.
Article in Plant physiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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31 citing papers in PubMed, 83 citations in OpenAlex.
- Opening the black box: in situ imaging of arbuscular mycorrhizal fungal structures in soil using synchrotron-based micro-CT.The New phytologist · 2026Article
- High-resolution microCT reveals relationships between stomata and interior leaf anatomy in sorghum.Plant physiology · 2026Article
- Phase-contrast microtomography unveils mechanisms of root colonization by a vascular fungal pathogen.Nature communications · 2026Article
- Improving 3D reconstruction quality for root phenotyping: assessing the impact of camera calibration and imaging parameters.Plant methods · 2026Article
- Root system architecture and drought adaptation: emerging tools and genetic insights.Frontiers in plant science · 2026Review
- Correlative x-ray microscopy and transmission electron microscopy for ultrastructural analysis of zebrafish tissue.Applied microscopy · 2025Article
- Plants in the expansion era: recent advances in expansion microscopy in plant systems.Journal of experimental botany · 2025Review
- Characterization of Grain Structure Using Micro-CT and Identification of Related Candidate Genes by QTL Mapping in Foxtail Millet.Plants (Basel, Switzerland) · 2025Article
- X-ray micro-CT imaging to study foliar water uptake mechanisms in plants with contrasting leaf topography.The New phytologist · 2025Article
- Panoptic segmentation for complete labeling of fruit microstructure in 3D micro-CT images with deep learning.Plant phenomics (Washington, D.C.) · 2025Article
- Best practices in plant fluorescence imaging and reporting: A primer.The Plant cell · 2025Review
- XFruitSeg-A general plant fruit segmentation model based on CT imaging.Plant phenomics (Washington, D.C.) · 2025Article
- Article
- The AMSlide for noninvasive time-lapse imaging of arbuscular mycorrhizal symbiosis.Journal of microscopy · 2025Article
- Evaluation of 3D seed structure and cellular traits in-situ using X-ray microscopy.Scientific reports · 2025Article
- Lattice light-sheet microscopy allows for super-resolution imaging of receptors in leaf tissue.Biophysical journal · 2025Article
- MRI-Seed-Wizard: combining deep learning algorithms with magnetic resonance imaging enables advanced seed phenotyping.Journal of experimental botany · 2025Article
- The pipelines of deep learning-based plant image processing.Quantitative plant biology · 2025Review
- CT image segmentation of foxtail millet seeds based on semantic segmentation model VGG16-UNet.Plant methods · 2024Article
- Non-destructive wood identification using X-ray µCT scanning: which resolution do we need?Plant methods · 2024Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Capturing complete internal anatomies of plant organs and tissues within their relevant morphological context remains a key challenge in plant science. While plant growth and development are inherently multiscale, conventional light, fluorescence, and electron microscopy platforms are typically limited to imaging of plant microstructure from small flat samples that lack a direct spatial context to, and represent only a small portion of, the relevant plant macrostructures. We demonstrate technical advances with a lab-based X-ray microscope (XRM) that bridge the imaging gap by providing multiscale high-resolution three-dimensional (3D) volumes of intact plant samples from the cell to the whole plant level. Serial imaging of a single sample is shown to provide sub-micron 3D volumes co-registered with lower magnification scans for explicit contextual reference. High-quality 3D volume data from our enhanced methods facilitate sophisticated and effective computational segmentation. Advances in sample preparation make multimodal correlative imaging workflows possible, where a single resin-embedded plant sample is scanned via XRM to generate a 3D cell-level map, and then used to identify and zoom in on sub-cellular regions of interest for high-resolution scanning electron microscopy. In total, we present the methodologies for use of XRM in the multiscale and multimodal analysis of 3D plant features using numerous economically and scientifically important plant systems.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.