ArticlePlant physiology2021
CRISPR/Cas9 mutants of tomato MICRORNA164 genes uncover their functional specialization in development.
Article in Plant physiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 48 citations in OpenAlex.
- Allopolyploidization-driven short-term evolution of miRNAs in Brassica A genome.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Sulfate Deficiency-Responsive MicroRNAs in Tomato Uncover an Expanded and Functionally Integrated Regulatory Network.International journal of molecular sciences · 2025Article
- Sugarcane microRNA shy-miR164 regulates sugar metabolism through direct cleavage of the transcription factor ScNAC mRNA.Plant physiology · 2025Article
- A SlRBP1-Horticulture research · 2025Article
- Streamlined protoplast transfection system for in-vivo validation and transgene-free genome editing in Banana.Transgenic research · 2025Article
- Role of non-coding RNAs in quality improvement of horticultural crops: computational tools, databases, and algorithms for identification and analysis.Functional & integrative genomics · 2025Review
- The molecular mechanism of leaf margin fission inFrontiers in plant science · 2025Article
- Detection of Ethylene Signaling Using Bioinformatics Tools in Plants.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Exploring the sheen: a review of research advances on fruit glossiness.Frontiers in plant science · 2025Review
- Divergence in MiRNA targeting of AchAco and its role in citrate accumulation in kiwifruit.BMC plant biology · 2024Article
- Identification and Analysis of theInternational journal of molecular sciences · 2024Article
- Recent Advances in Studying the Regulation of Fruit Ripening in Tomato Using Genetic Engineering Approaches.International journal of molecular sciences · 2024Review
- Review
- MicroRNAs associated with AGL6 and IAA9 function in tomato fruit set.BMC research notes · 2023Article
- Coordinating Diverse Functions of miRNA and lncRNA in Fleshy Fruit.Plants (Basel, Switzerland) · 2023Review
- Multiple Functions of MiRNAs inLife (Basel, Switzerland) · 2022Review
- Tomato MicroRNAs and Their Functions.International journal of molecular sciences · 2022Review
- CLASS-II KNOX genes coordinate spatial and temporal ripening in tomato.Plant physiology · 2022Article
- SlMIR164A regulates fruit ripening and quality by controlling SlNAM2 and SlNAM3 in tomato.Plant biotechnology journal · 2022Article
- Integrative analysis of transcriptome and miRNAome reveals molecular mechanisms regulating pericarp thickness in sweet corn during kernel development.Frontiers in plant science · 2022Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant MICRORNA164 (miR164) plays diverse regulatory functions by post-transcriptional repression of certain NAM/ATAF/CUC-domain transcription factors. However, the involvement of miR164 in fleshy fruit development and ripening remains poorly understood. Here, de novo prediction of tomato (Solanum lycopersicum) MIR164 genes identified four genes (SlMIR164a-d), of which SlMIR164d has an atypically long pre-miRNA. The roles of the fruit expressed SlMIR164a, b, and d were studied by analysis of their Clustered Regularly Interspaced Short Palindromic Repeats mutants. The slmir164bCR mutant plants exhibited shoot and flower abnormalities characteristic of ectopic boundary specification, whereas the shoot and flower development of slmir164aCR and slmir164dCR mutants were indistinguishable from wild-type. Strikingly, the knockout of SlMIR164a practically eliminated sly-miR164 from the developing and ripening fruit pericarp. The sly-miR164-deficient slmir164aCR fruits were smaller than the wild-type, due to reduced pericarp cell division and expansion, and displayed intense red color and matte, instead of glossy appearance, upon ripening. We found that the fruit skin phenotypes were associated with morphologically abnormal outer epidermis and thicker cuticle. Quantitation of sly-miR164 target transcripts in slmir164aCR ripening fruits demonstrated the upregulation of SlNAM3 and SlNAM2. Specific expression of their miR164-resistant versions in the pericarp resulted in the formation of extremely small fruits with abnormal epidermis, highlighting the importance of their negative regulation by sly-miR164a. Taken together, our results demonstrate that SlMIR164a and SlMIR164b play specialized roles in development: SlMIR164b is required for shoot and flower boundary specification, and SlMIR164a is required for fruit growth including the expansion of its outer epidermis, which determines the properties of the fruit skin.
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