ArticlePlant physiology2021
Machine learning-enabled phenotyping for GWAS and TWAS of WUE traits in 869 field-grown sorghum accessions.
Article in Plant physiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
46 citing papers in PubMed, 1 synthesis or guideline pooled it, 96 citations in OpenAlex.
- Multi-omics revolution to promote plant breeding efficiency.Frontiers in plant science · 2022Pooled it
- Improving multi-trait genomic prediction using synthetic traits from hyperspectral data based on co-heritability.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Beyond the Classics: The Synergy of AI and Genomics Reveals an Expanded Repertoire of Pigmentation Genes.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2026Review
- Genome-wide association studies and modeling of stomatal gas conductance reveal genetic control of water-use efficiency in sorghum.Plant physiology · 2026Article
- Virus-induced gene editing of stomatal regulators in Nicotiana benthamiana enables rapid functional genomics.The Plant journal : for cell and molecular biology · 2026Article
- Integrating natural variation through GWAS - genetics of drought and flood tolerance in grass pea reveal independent yet interconnected mechanisms.BMC plant biology · 2026Article
- High-Throughput Screen of NPQ in Sorghum Shows Highly Polygenic Architecture of Photoprotection.Plant-environment interactions (Hoboken, N.J.) · 2026Article
- Candidate genes for anthracnose resistance in Senegalese sorghum: a machine learning-based exploration.Functional & integrative genomics · 2025Article
- Stomata in-sight: Integrating live confocal microscopy with leaf gas exchange and environmental control.Plant physiology · 2025Article
- Article
- Smarter stomata: emergent technologies unlocking yield potential in a changing climate.AoB PLANTS · 2025Review
- Translational insights into abiotic interactions: From Arabidopsis to crop plants.The Plant cell · 2025Review
- Genes and pathways determining flowering time variation in temperate-adapted sorghum.The Plant journal : for cell and molecular biology · 2025Article
- Integrative omics analysis reveals the genetic basis of fatty acid composition in Brassica napus seeds.Genome biology · 2025Article
- A deficient CP24 allele defines variation for dynamic nonphotochemical quenching and photosystem II efficiency in maize.The Plant cell · 2025Article
- Comparative transcriptome analysis identified candidate genes associated with kernel row number in maize.PeerJ · 2025Article
- Machine learning-enabled computer vision for plant phenotyping: a primer on AI/ML and a case study on stomatal patterning.Journal of experimental botany · 2024Review
- Reducing stomatal density by expression of a synthetic epidermal patterning factor increases leaf intrinsic water use efficiency and reduces plant water use in a C4 crop.Journal of experimental botany · 2024Article
- Stomatal development in the changing climate.Development (Cambridge, England) · 2024Review
- TWAS facilitates gene-scale trait genetic dissection through gene expression, structural variations, and alternative splicing in soybean.Plant communications · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sorghum (Sorghum bicolor) is a model C4 crop made experimentally tractable by extensive genomic and genetic resources. Biomass sorghum is studied as a feedstock for biofuel and forage. Mechanistic modeling suggests that reducing stomatal conductance (gs) could improve sorghum intrinsic water use efficiency (iWUE) and biomass production. Phenotyping to discover genotype-to-phenotype associations remains a bottleneck in understanding the mechanistic basis for natural variation in gs and iWUE. This study addressed multiple methodological limitations. Optical tomography and a machine learning tool were combined to measure stomatal density (SD). This was combined with rapid measurements of leaf photosynthetic gas exchange and specific leaf area (SLA). These traits were the subject of genome-wide association study and transcriptome-wide association study across 869 field-grown biomass sorghum accessions. The ratio of intracellular to ambient CO2 was genetically correlated with SD, SLA, gs, and biomass production. Plasticity in SD and SLA was interrelated with each other and with productivity across wet and dry growing seasons. Moderate-to-high heritability of traits studied across the large mapping population validated associations between DNA sequence variation or RNA transcript abundance and trait variation. A total of 394 unique genes underpinning variation in WUE-related traits are described with higher confidence because they were identified in multiple independent tests. This list was enriched in genes whose Arabidopsis (Arabidopsis thaliana) putative orthologs have functions related to stomatal or leaf development and leaf gas exchange, as well as genes with nonsynonymous/missense variants. These advances in methodology and knowledge will facilitate improving C4 crop WUE.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.