ArticlePlant physiology2021
Mitochondrial carbonic anhydrases are needed for optimal photosynthesis at low CO2 levels in Chlamydomonas.
Article in Plant physiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 32 citations in OpenAlex.
- The mosaic of aquatic photorespiration: evolution, diversity, and global biogeochemical impacts.The New phytologist · 2026Review
- Sensitivity of Photosystem II to Photoinhibition inInternational journal of molecular sciences · 2026Article
- CIA5 and its interacting metal-binding GTPase ZNG3 are degraded by the proteasome in Zn deficiency.The Plant journal : for cell and molecular biology · 2026Article
- Mitochondrial support of high rates of photosynthesis.Plant communications · 2025Article
- Light-dark dependent changes in chloroplast and mitochondrial activity inFrontiers in plant science · 2025Article
- Highly Active Carbonic Anhydrase of the Thylakoid Lumen ofPlants (Basel, Switzerland) · 2024Article
- Dramatic changes in mitochondrial subcellular location and morphology accompany activation of the COProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Dramatic Changes in Mitochondrial Subcellular Location and Morphology Accompany Activation of the CObioRxiv : the preprint server for biology · 2024Article
- CAH3 fromCells · 2024Review
- Visualizing the dynamics of plant energy organelles.Biochemical Society transactions · 2023Article
- A pyrenoid-localized protein SAGA1 is necessary for CaPhotosynthesis research · 2023Article
- Adapting from Low to High: An Update to COPlants (Basel, Switzerland) · 2023Review
- The Influence of Symbiosis on the Proteome of theMicroorganisms · 2023Article
- A Rapid Method for Detecting Normal or Modified Plant and Algal Carbonic Anhydrase Activity UsingPlants (Basel, Switzerland) · 2022Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chlamydomonas reinhardtii can grow photosynthetically using CO2 or in the dark using acetate as the carbon source. In the light in air, the CO2 concentrating mechanism (CCM) of C. reinhardtii accumulates CO2, enhancing photosynthesis. A combination of carbonic anhydrases (CAs) and bicarbonate transporters in the CCM of C. reinhardtii increases the CO2 concentration at Ribulose 1,5-bisphosphate carboxylase oxygenase (Rubisco) in the chloroplast pyrenoid. Previously, CAs important to the CCM have been found in the periplasmic space, surrounding the pyrenoid and inside the thylakoid lumen. Two almost identical mitochondrial CAs, CAH4 and CAH5, are also highly expressed when the CCM is made, but their role in the CCM is not understood. Here, we adopted an RNAi approach to reduce the expression of CAH4 and CAH5 to study their possible physiological functions. RNAi mutants with low expression of CAH4 and CAH5 had impaired rates of photosynthesis under ambient levels of CO2 (0.04% CO2 [v/v] in air). These strains were not able to grow at very low CO2 (<0.02% CO2 [v/v] in air), and their ability to accumulate inorganic carbon (Ci = CO2 + HCO3-) was reduced. At low CO2 concentrations, the CCM is needed to both deliver Ci to Rubisco and to minimize the leak of CO2 generated by respiration and photorespiration. We hypothesize that CAH4 and CAH5 in the mitochondria convert the CO2 released from respiration and photorespiration as well as the CO2 leaked from the chloroplast to HCO3- thus "recapturing" this potentially lost CO2.
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