ArticleMolecular plant pathology2022
Nicotiana benthamiana asparagine synthetase associates with IP-L and confers resistance against tobacco mosaic virus via the asparagine-induced salicylic acid signalling pathway.
Article in Molecular plant pathology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 25 citations in OpenAlex.
- Asparagine Synthetase 1 regulates rice seminal root elongation via jasmonic acid metabolism.Plant cell reports · 2026Article
- Polyglutamate-loaded chitosan nanogels reprogram plant metabolism for increased growth and viral resistance.Nature communications · 2026Article
- GhLYK5-mediated GhNIT4B activates asparagine and ROS accumulation enhancing cotton Verticillium wilt resistance.Journal of biological engineering · 2026Article
- The Multifaceted Functions of Plant Asparagine Synthetase: Regulatory Mechanisms and Functional Diversity in Growth and Defense.Plants (Basel, Switzerland) · 2026Review
- Combined transcriptome and metabolome analyses reveal the effects of selenium on the growth and quality ofFrontiers in plant science · 2024Article
- Size variations of mesoporous silica nanoparticle control uptake efficiency and delivery of AC2-derived dsRNA for protection against tomato leaf curl New Delhi virus.Plant cell reports · 2023Article
- Uncovering genomic and transcriptional variations facilitates utilization of wild resources in cotton disease resistance improvement.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Article
- AInternational journal of molecular sciences · 2022Article
- Nicotiana benthamiana asparagine synthetase associates with IP-L and confers resistance against tobacco mosaic virus via the asparagine-induced salicylic acid signalling pathway.Molecular plant pathology · 2022Article
- Phytohormones and emerging plant growth regulators in tailoring plant immunity against viral infections.Physiologia plantarumReview
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Asparagine synthetase is a key enzyme that catalyses the conversion of amide groups from glutamine or ammonium to aspartate, which leads to the generation of asparagine. However, the role of asparagine synthetase in plant immunity remains largely unknown. Here, we identified a Nicotiana benthamiana asparagine synthetase B (NbAS-B) that associates with tomato mosaic virus coat protein-interacting protein L (IP-L) using the yeast two-hybrid assay and examined its role in tobacco mosaic virus (TMV) resistance. The association of IP-L with NbAS-B was further confirmed by in vivo co-immunoprecipitation, luciferase complementation imaging, and bimolecular fluorescence complementation assays. IP-L and NbAS-B interact in the nucleus and cytosol and IP-L apparently stabilizes NbAS-B, thus enhancing its accumulation. The expressions of IP-L and NbAS-B are continuously induced on TMV-green fluorescent protein (GFP) infection. Co-silencing of IP-L and NbAS-B facilitates TMV-GFP infection. Overexpression of NbAS-B in tobacco reduces TMV-GFP infection by significantly improving the synthesis of asparagine. Furthermore, the external application of asparagine significantly inhibits the infection of TMV-GFP by activating the salicylic acid signalling pathway. These findings hold the potential for the future application of asparagine in the control of TMV.
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