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ArticleMolecular biology reports2021

Isolation of microsatellite markers for the endemic Phlomis lychnitis (Lamiaceae).

Paloma Triguero-Piñero, Clara de Vega, Abelardo Aparicio, Rafael G Albaladejo

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Article in Molecular biology reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
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3 · Its place in the literature

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1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

4 authors.

Paloma Triguero-PiñeroDepartamento de Biología Vegetal y Ecología, Facultad de Farmacia, Universidad de Sevilla, c/Profesor García González no 2, 41012, Seville, Spain.
Clara de VegaDepartamento de Biología Vegetal y Ecología, Facultad de Farmacia, Universidad de Sevilla, c/Profesor García González no 2, 41012, Seville, Spain.ORCID http://orcid.org/0000-0003-4553-798X
Abelardo AparicioDepartamento de Biología Vegetal y Ecología, Facultad de Farmacia, Universidad de Sevilla, c/Profesor García González no 2, 41012, Seville, Spain.ORCID https://orcid.org/0000-0001-7122-4421
Rafael G AlbaladejoDepartamento de Biología Vegetal y Ecología, Facultad de Farmacia, Universidad de Sevilla, c/Profesor García González no 2, 41012, Seville, Spain. albaladejo@us.es.ORCID https://orcid.org/0000-0003-2101-5204

Funding

consejería de economía, innovación, ciencia y empleo, junta de andalucía Ayuda a Grupos de Investigación grupos PAIDI RNM-210
6 · The paper itself

Abstract

backgroundPhlomis lychnitis is a mostly endemic species of the Iberian Peninsula that frequently hybridizes with the narrow endemic P. crinita subsp. malacitana in southern Spain. Whenever they coexist they form homoploid hybrid zones. Unlike hybridization at the polyploid level, the process of hybridization at the homoploid level is much less well known. METHODS AND

resultsIn this study we report the development of 22 microsatellite markers through next-generation sequencing technologies for P. lychnitis. We characterize the genetic diversity for two populations of this species for the 10 markers that resulted to be polymorphic. Further, we check the transferability of these polymorphic markers to one population of P. crinita subsp. malacitana to verify the potential use of these markers for hybridization studies. The values of expected heterozygosity for P. lychnitis were higher than in P. crinita subsp. malacitana, and the three analyzed populations displayed negative values for the inbreeding coefficient which is compatible with the frequent instances of hybridization and introgression between species.

conclusionsThis set of polymorphic markers are useful for further studies aiming at a deeper understanding of the homoploid hybrid process between these species. Additionally, this is the first panel of microsatellite markers developed for the genus Phlomis, a genus very rich in endemic species and with medicinal properties that could benefit from the use of these new markers.

Indexed as

DNAGenetic VariationHeterozygoteHigh-Throughput Nucleotide SequencingHybridization, GeneticLamiaceaeMicrosatellite RepeatsPhlomisPolyploidySequence Analysis, DNASpainDNAHomoploid hybridizationIntrogressionMicrosatellitesPhlomisPopulation genetics

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.