ArticleScientific reports2021
Identification of candidate genes from androgenic gland in Macrobrachium nipponense regulated by eyestalk ablation.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 29 citations in OpenAlex.
- The Effects of Eyestalk Ablation on the Androgenic Gland and the Male Reproductive Organs in the Kuruma PrawnAnimals : an open access journal from MDPI · 2025Article
- Impact of Salinity Stress on Antioxidant Enzyme Activity, Histopathology, and Gene Expression in the Hepatopancreas of the Oriental River Prawn,Animals : an open access journal from MDPI · 2025Article
- Investigation of the Effects of Salinity Exposure on Immune Defense, Morphology, and Gene Expression in the Gills ofAntioxidants (Basel, Switzerland) · 2025Article
- Transcriptome Profiling Analysis Reveals Changes in the Antioxidant Defense System, Morphology, and Gene Expression in the Gills ofInternational journal of molecular sciences · 2025Article
- Identification of the effects of alkalinity exposure on the gills of oriental river prawns, Macrobrachium nipponense.BMC genomics · 2024Article
- Identifying Relationships betweenAnimals : an open access journal from MDPI · 2024Article
- Identification of Candidate Male-Reproduction-Related Genes from the Testis and Androgenic Gland ofInternational journal of molecular sciences · 2024Article
- Transcriptome analysis of reproductive tract tissues of male river prawn Macrobrachium americanum.Molecular biology reports · 2024Article
- Effects of Alkalinity Exposure on Antioxidant Status, Metabolic Function, and Immune Response in the Hepatopancreas ofAntioxidants (Basel, Switzerland) · 2024Article
- Temperature-Induced Sex Differentiation in River Prawn (International journal of molecular sciences · 2024Article
- Effects of Dietary Folic Acid Supplementation on Sex Differences in Oriental River Prawn,Animals : an open access journal from MDPI · 2023Article
- RNAi Analysis of Potential Functions of Cyclin B3 in Reproduction of Male Oriental River Prawns (Animals : an open access journal from MDPI · 2023Article
- Molecular Characterization Related to Ovary Early Development Mechanisms after Eyestalk Ablation inBiology · 2023Article
- Article
- Identification and Expression Analysis ofInternational journal of molecular sciences · 2022Article
- Identification of Important Genes Involved in the Sex-Differentiation Mechanism of Oriental River Prawn,Frontiers in genetics · 2022Article
- Identification of potential functions of polo-like kinase 1 in male reproductive development of the oriental river prawn (Frontiers in endocrinology · 2022Article
- RNA interference analysis of potential functions of cyclin A in the reproductive development of male oriental river prawns (Frontiers in genetics · 2022Article
- Effects of Luteinizing Hormone Releasing Hormone A2 on Gonad Development in Juvenile Amur Sturgeon, Acipenser schrenckii, Revealed by Transcriptome Profiling Analysis.Frontiers in genetics · 2022Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The eyestalk of crustaceans, such as Macrobrachium nipponense, contains many neurosecretory hormones affecting the process of reproduction, molting, metabolism of glucose, and other functions. In this study, important metabolic pathways and candidate genes involved in male sexual development were selected from M. nipponense. The methodology involved performing long-read and next generation transcriptome sequencing of genes from the androgenic gland after eyestalk ablation. qPCR analysis revealed that the mRNA expression of Mn-IAG was significantly increased after ablation of both the single-side (SS) and double-side (DS) eyestalk, compared with the control group (CG). The long-read transcriptome generated 49,840 non-redundant transcripts. A total of 1319, 2092 and 4351 differentially expressed genes (DEGs) were identified between CG versus SS, SS versus DS and CG versus DS, respectively. These data indicated that ablation of the double-sided eyestalk played stronger regulatory roles than the single-side ablation on male sexual development in M. nipponense. This was consistent with the qPCR analysis. Cell Cycle, Cellular Senescence, Oxidative Phosphorylation, Glycolysis/Gluconeogenesis and Steroid Hormone Biosynthesis were the primary enriched metabolic pathways in all three comparisons, and the important genes from these metabolic pathways were also selected. qPCR permitted secondary confirmation of ten DEGs identified through RNA-seq. RNAi-mediated silencing analyses of Hydroxysteroid dehydrogenase like 1 (HSDL1) revealed that HSDL1 has a positive regulatory effect on testes development. This study provides valuable insight into male sexual development in M. nipponense, including metabolic pathways and genes, paving the way for advanced studies on male sexual development in this species and in other crustaceans.
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