Evidence map›Paper›PMID 34615385›Full record

ArticleCancer control : journal of the Moffitt Cancer Center

Peripheral Blood-Based DNA Methylation of Long Non-Coding RNA H19 and Metastasis-Associated Lung Adenocarcinoma Transcript 1 Promoters are Potential Non-Invasive Biomarkers for Gastric Cancer Detection.

Dingtao Hu, Xiaoqi Lou, Nana Meng, Zhen Li, Ying Teng, Yanfeng Zou, Fang Wang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer control : journal of the Moffitt Cancer Center. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.1field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 16 citations in OpenAlex.

  1. Association ofTranslational cancer research · 2026
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  7. Novel biomarkers for early detection of gastric cancer.World journal of gastroenterology · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Dingtao HuDepartment of Oncology, 36639The First Affiliated Hospital of Anhui Medical University, China.
Xiaoqi LouDepartment of Oncology, 36639The First Affiliated Hospital of Anhui Medical University, China.
Nana MengDepartment of Quality Management Office, 533251The Second Affiliated Hospital of Anhui Medical University, China.
Zhen LiDepartment of Epidemiology and Biostatistics, School of Public Health of Anhui Medical University, China.
Ying TengDepartment of Epidemiology and Biostatistics, School of Public Health of Anhui Medical University, China.
Yanfeng ZouDepartment of Epidemiology and Biostatistics, School of Public Health of Anhui Medical University, China.
Fang WangDepartment of Oncology, 36639The First Affiliated Hospital of Anhui Medical University, China.ORCID https://orcid.org/0000-0003-0871-780X
Anhui Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionThe early diagnosis and detection could greatly improve the clinical outcome of gastric cancer (GC) patients. However, the non-invasive biomarkers for GC detection remain to be identified.

methodWe used online databases (GEPIA, UALCAN, Kaplan-Meier plotter, TIMER, and MEXPRESS) to explore the association between H19 or metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) expression in tissues and the occurrence, development, prognosis, the levels of immune cell infiltration, and methylation of GC; the correlation between mRNA expression and DNA methylation levels of genes were also examined. Methylation levels of H19 or MALAT1 in peripheral blood were compared between 150 GC patients and 100 healthy controls (HCs). Predictive nomograms were constructed among female and male groups for GC diagnosis. The calibration curves, Hosmer-Lemeshow test, and decision curve analysis were also used to examine the nomograms' predictive ability and clinical values.

resultsUsing multiple online databases, we found that the mRNA expressions of H19 and MALAT1 in tissues were related to the occurrence of GC, and such expressions were associated with immune cell infiltration of GC and negatively correlated with DNA methylation levels of H19 and MALAT1. H19 gene, H19C island, and MALAT1B island, as well as 20 CpG sites were hypermethylated in peripheral blood of GC patients compared with HCs; similar results were also found in female and male groups (

conclusionWe found statistically significant hypermethylation of H19 and MALAT1 promoters in GC patients, and meaningful sensitivity and specificity of MALAT1 and H19 methylation in discriminating GC and HCs were observed in both female and male groups, which indicates that the peripheral blood-based DNA methylation of H19 and MALAT1 could act as potential non-invasive biomarkers for the diagnosis of GC.

Indexed as

Biomarkers, TumorBody Mass IndexChinaDNA MethylationFemaleGene Expression Regulation, NeoplasticHumansMalePrognosisPromoter Regions, GeneticRNA, Long NoncodingSensitivity and SpecificitySex FactorsStomach NeoplasmsBiomarkers, TumorH19 long non-coding RNAMALAT1 long non-coding RNA, humanRNA, Long NoncodingDNA methylationgastric cancerH19metastasis-associated lung adenocarcinoma transcript 1peripheral blood

Identifiers

PMID34615385
PMCPMC8504648
OpenAlexW3204574971

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.