Evidence map›Paper›PMID 34614184›Full record

ArticleNucleic acids research2021

Binding of DNA origami to lipids: maximizing yield and switching via strand displacement.

Jasleen Kaur Daljit Singh, Esther Darley, Pietro Ridone, James P Gaston, Ali Abbas, Shelley F J Wickham, Matthew A B Baker

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nucleic acids research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
2.1field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 33 citations in OpenAlex.

  1. DNA-directed assembly of multivalent lipid nanoparticles for targeted T cell gene delivery.Journal of controlled release : official journal of the Controlled Release Society · 2026
    Article
  2. Article
  3. DNA Origami Vesicle Sensors with Triggered Single-Molecule Cargo Transfer.Angewandte Chemie (International ed. in English) · 2024
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Jasleen Kaur Daljit SinghSchool of Chemistry, The University of Sydney, Sydney, New South Wales, Australia.
Esther DarleySchool of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia.
Pietro RidoneSchool of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia.
James P GastonSchool of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia.
Ali AbbasSchool of Chemical and Biomolecular Engineering, The University of Sydney, Sydney, New South Wales, Australia.
Shelley F J WickhamSchool of Chemistry, The University of Sydney, Sydney, New South Wales, Australia.
Matthew A B BakerSchool of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia.ORCID 0000-0002-5839-6904
The University of Sydney · AUUNSW Sydney · AUCommonwealth Scientific and Industrial Research Organisation · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Liposomes are widely used as synthetic analogues of cell membranes and for drug delivery. Lipid-binding DNA nanostructures can modify the shape, porosity and reactivity of liposomes, mediated by cholesterol modifications. DNA nanostructures can also be designed to switch conformations by DNA strand displacement. However, the optimal conditions to facilitate stable, high-yield DNA-lipid binding while allowing controlled switching by strand displacement are not known. Here, we characterized the effect of cholesterol arrangement, DNA structure, buffer and lipid composition on DNA-lipid binding and strand displacement. We observed that binding was inhibited below pH 4, and above 200 mM NaCl or 40 mM MgCl2, was independent of lipid type, and increased with membrane cholesterol content. For simple motifs, binding yield was slightly higher for double-stranded DNA than single-stranded DNA. For larger DNA origami tiles, four to eight cholesterol modifications were optimal, while edge positions and longer spacers increased yield of lipid binding. Strand displacement achieved controlled removal of DNA tiles from membranes, but was inhibited by overhang domains, which are used to prevent cholesterol aggregation. These findings provide design guidelines for integrating strand displacement switching with lipid-binding DNA nanostructures. This paves the way for achieving dynamic control of membrane morphology, enabling broader applications in nanomedicine and biophysics.

Indexed as

CholesterolDNADNA, Single-StrandedHydrogen-Ion ConcentrationKineticsLiposomesMagnesium ChlorideNanostructuresNucleic Acid ConformationPhosphatidylcholinesPhosphatidylethanolaminesSodium ChlorideSolutionsThermodynamics1,2-diphytanoylphosphatidylcholine1,2-oleoylphosphatidylcholineCholesteroldioleoyl phosphatidylethanolamineDNADNA, Single-StrandedLiposomesMagnesium ChloridePhosphatidylcholinesPhosphatidylethanolaminesSodium ChlorideSolutions

Identifiers

PMID34614184
PMCPMC8565350
OpenAlexW3202347243

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.