Evidence map›Paper›PMID 34614151›Full record

ArticleThe Journal of cell biology2021

Subunit cooperation in the Get1/2 receptor promotes tail-anchored membrane protein insertion.

Un Seng Chio, Yumeng Liu, SangYoon Chung, Woo Jun Shim, Sowmya Chandrasekar, Shimon Weiss, Shu-Ou Shan

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of cell biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.4field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Un Seng Chio *Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA.ORCID 0000-0002-5295-2690
Yumeng Liu *Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA.
SangYoon ChungDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA.
Woo Jun ShimDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA.
Sowmya ChandrasekarDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA.
Shimon WeissDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA.ORCID 0000-0002-0720-5426
Shu-Ou ShanDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA.ORCID 0000-0002-6526-1733
California Institute of Technology · USBar-Ilan University · ILUniversity of California, Los Angeles · US

Funding

Supplement: Accurate Molecular Decision Making during Protein BiogenesisR35GM136321 · NIGMS · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI Shu-ou Shan · 2020 to 2026
$6.7M
Molecular mechanisms of post-translational targeting of tail-anchored proteins.R01GM107368 · NIGMS · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI SHAN, SHU-OU · 2014 to 2019
$2.1M
NIGMS NIH HHS R01 GM107368NIGMS NIH HHS R35 GM136321NIH HHS R01 GM107368
6 · The paper itself

Abstract

The guided entry of tail-anchored protein (GET) pathway, in which the Get3 ATPase delivers an essential class of tail-anchored membrane proteins (TAs) to the Get1/2 receptor at the endoplasmic reticulum, provides a conserved mechanism for TA biogenesis in eukaryotic cells. The membrane-associated events of this pathway remain poorly understood. Here we show that complex assembly between the cytosolic domains (CDs) of Get1 and Get2 strongly enhances the affinity of the individual subunits for Get3•TA, thus enabling efficient capture of the targeting complex. In addition to the known role of Get1CD in remodeling Get3 conformation, two molecular recognition features (MoRFs) in Get2CD induce Get3 opening, and both subunits are required for optimal TA release from Get3. Mutation of the MoRFs attenuates TA insertion into the ER in vivo. Our results demonstrate extensive cooperation between the Get1/2 receptor subunits in the capture and remodeling of the targeting complex, and emphasize the role of MoRFs in receptor function during membrane protein biogenesis.

Indexed as

Adaptor Proteins, Vesicular TransportCytosolEndoplasmic ReticulumMembrane ProteinsMutationProtein BiosynthesisProtein TransportSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsAdaptor Proteins, Vesicular TransportGET1 protein, S cerevisiaeGet2 protein, S cerevisiaeMembrane ProteinsSaccharomyces cerevisiae Proteins

Identifiers

PMID34614151
PMCPMC8530227
OpenAlexW3203760916

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.