ArticleApplied and environmental microbiology2021
Identification of Copper-Containing Oxidoreductases in the Secretomes of Three
Article in Applied and environmental microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 24 citations in OpenAlex.
- A time-course analysis ofApplied and environmental microbiology · 2024Article
- Expansion of Auxiliary Activity Family 5 sequence space via biochemical characterization of six new copper radical oxidases.Applied and environmental microbiology · 2024Article
- Methionine oxidation of carbohydrate-active enzymes during white-rot wood decay.Applied and environmental microbiology · 2024Article
- Genome evolution and transcriptome plasticity is associated with adaptation to monocot and dicot plants in Colletotrichum fungi.GigaScience · 2024Article
- Effective biosynthesis of 2,5-furandicarboxylic acid from 5-hydroxymethylfurfural via a bi-enzymatic cascade system using bacterial laccase and fungal alcohol oxidase.Biotechnology for biofuels and bioproducts · 2023Article
- Enzymatic reactions towards aldehydes: An overview.Flavour and fragrance journal · 2023Review
- A simple and direct ionic chromatography method to monitor galactose oxidase activity.RSC advances · 2022Article
- Copper-radical oxidases: A diverse group of biocatalysts with distinct properties and a broad range of biotechnological applications.Engineering microbiology · 2022Review
- Active-Site Engineering Switches Carbohydrate Regiospecificity in a Fungal Copper Radical Oxidase.ACS catalysis · 2022Article
- Tunable Production of (ACS catalysis · 2022Article
- A LignocellulolyticJournal of fungi (Basel, Switzerland) · 2021Article
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Authors and funding
14 authors at 4 institutions in 2 countries.
Funding
Abstract
Copper radical alcohol oxidases (CRO-AlcOx), which have been recently discovered among fungal phytopathogens, are attractive for the production of fragrant fatty aldehydes. With the initial objective to investigate the secretion of CRO-AlcOx by natural fungal strains, we undertook time course analyses of the secretomes of three Colletotrichum species (C. graminicola, C. tabacum, and C. destructivum) using proteomics. The addition of a copper-manganese-ethanol mixture in the absence of any plant-biomass mimicking compounds to Colletotrichum cultures unexpectedly induced the secretion of up to 400 proteins, 29 to 52% of which were carbohydrate-active enzymes (CAZymes), including a wide diversity of copper-containing oxidoreductases from the auxiliary activities (AA) class (AA1, AA3, AA5, AA7, AA9, AA11, AA12, AA13, and AA16). Under these specific conditions, while a CRO-glyoxal oxidase from the AA5_1 subfamily was among the most abundantly secreted proteins, the targeted AA5_2 CRO-AlcOx were secreted at lower levels, suggesting heterologous expression as a more promising strategy for CRO-AlcOx production and utilization. C. tabacum and C. destructivum CRO-AlcOx were thus expressed in Pichia pastoris, and their preference toward both aromatic and aliphatic primary alcohols was assessed. The CRO-AlcOx from C. destructivum was further investigated in applied settings, revealing a full conversion of C
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