ArticleInternational journal of experimental pathology2021
A simple optical tissue clearing pipeline for 3D vasculature imaging of the mediastinal organs in mice.
Article in International journal of experimental pathology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Aging disrupts sympathetic innervation of the thymus.Cell reports · 2026Article
- Identifying space-resolved proteins of the murine thymus, by combining MALDI-MSI and proteomics.Life science alliance · 2026Article
- Ethyl Cinnamate-Based Tissue Clearing Technique on Vocal Fold Mucosa with Vasculature Model.Journal of voice : official journal of the Voice Foundation · 2025Article
- Progress on three-dimensional visualizing skin architecture with multiple immunofluorescence staining and tissue-clearing approaches.Histology and histopathology · 2025Review
- Three-dimensional exploration of the chicken embryo, a comparative study of light sheet and histological visualisation.PloS one · 2025Article
- Neural regulation of the thymus: past, current, and future perspectives.Frontiers in immunology · 2025Review
- Completing the view - histologic insights from circular AAA specimen including 3D imaging : A methodologic approach towards histologic analysis of circumferential AAA samples.Diagnostic pathology · 2023Article
- Bidirectional crosstalk between the peripheral nervous system and lymphoid tissues/organs.Frontiers in immunology · 2023Review
- A simple optical tissue clearing pipeline for 3D vasculature imaging of the mediastinal organs in mice.International journal of experimental pathology · 2021Article
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Authors and funding
5 authors.
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Abstract
Optical tissue clearing (OTC) methods render tissue transparent by matching the refractive index within a sample to enable three-dimensional (3D) imaging with advanced microscopes. The application of OTC method in mediastinal organs in mice remains poorly understand. Our aim was to establish a simple protocol pipeline for 3D imaging of the mediastinal organs in mice. Trachea, oesophagus, thymus and heart were harvested from mice after retrograde perfusion via the abdominal aorta. We combined and optimized antibody labelling of thick tissue samples, OTC with cheap and non-toxic solvent ethyl cinnamate (ECi), and light-sheet fluorescence microscopy (LSFM) or laser confocal fluorescence microscopy (LCFM) to visualize the vasculature of those tissues. A high degree of optical transparency of trachea, oesophagus, thymus and heart was achieved after ECi-based OTC. With anti-CD31 antibody immunofluorescence labelling before ECi-based OTC, the vasculature of these tissues with their natural morphology, location and organizational network was imaged using LSFM or LCFM. This simple protocol pipeline provides an easy-to-setup and comprehensive way to study the vasculature of mediastinal organs in 3D without any special equipment. We anticipate that it will facilitate diverse applications in biomedical research of thoracic diseases and even other organs.
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