ArticlemSphere2021
Temperature Influences the Composition and Cytotoxicity of Extracellular Vesicles in Staphylococcus aureus.
Article in mSphere, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
33 citing papers in PubMed, 44 citations in OpenAlex.
- Towards a Scalable Production ofInternational journal of molecular sciences · 2026Article
- Group B streptococcal membrane vesicles induce proinflammatory responses in neonatal meninges.Infection and immunity · 2026Article
- Integrated multi-omics profiling of gut bacterial extracellular vesicles links cargo composition to host transcriptional responses.bioRxiv : the preprint server for biology · 2026Article
- Bacterial extracellular membrane vesicles as multifunctional defense systems: Roles in immune evasion, phage interactions, and antimicrobial resistance.Molecular biology reports · 2026Review
- Bacterial Extracellular Vesicles in the Strategic Interplay Between Pathogens and Hosts.Microorganisms · 2026Review
- Staphylococcus aureus derived extracellular vesicles modulate osteoblast-like cell immune responses independently of vesicle internalization.Scientific reports · 2026Article
- Environmental cues shape extracellular vesicles biogenesis and function in Streptococcus pneumoniae.BMC microbiology · 2026Article
- Normoxic stress enhance the secretion of adhesion-promoting membrane vesicles byJournal of oral microbiology · 2026Article
- Nasal microbionts differentially colonize and elicit cytokines in human nasal epithelial organoids.mSphere · 2025Article
- The Role of Oral and Gut Microbiota in Bone Health: Insights from Bacterial Extracellular Vesicles.Microorganisms · 2025Review
- Optimizing Extracellular Products from Vibrio proteolyticus for Their Use as Postbiotics in Aquaculture.Marine biotechnology (New York, N.Y.) · 2025Article
- Beta-Lactam Antibiotics Promote Extracellular Vesicle Production of Staphylococcus aureus Through ROS-Mediated Lipid Metabolic Reprogramming.Journal of extracellular vesicles · 2025Article
- Lipid metabolism, remodelling and intercellular transfer in the CNS.Nature reviews. Neuroscience · 2025Review
- Unveiling the wonders of bacteria-derived extracellular vesicles: From fundamental functions to beneficial applications.Heliyon · 2025Review
- Sub-inhibitory concentrations of oxacillin modulate biogenesis and function of extracellular vesicles secreted by oxacillin-sensitive methicillin-resistantFrontiers in microbiology · 2025Article
- Staphylococcus aureus membrane vesicles: an evolving story.Trends in microbiology · 2024Review
- The Role of Bacterial Extracellular Vesicles in the Immune Response to Pathogens, and Therapeutic Opportunities.International journal of molecular sciences · 2024Review
- TA-Cloning for Diabetes Treatment: Expressing Corynebacterium Malic Enzyme Gene in E. coli.Current microbiology · 2024Article
- Evaluation of the Differential Postbiotic Potential of Shewanella putrefaciens Pdp11 Cultured in Several Growing Conditions.Marine biotechnology (New York, N.Y.) · 2024Article
- RNA-containing extracellular vesicles in infection.RNA biology · 2024Review
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
Staphylococcus aureus is a pathogenic bacterium but also a commensal of skin and anterior nares in humans. As S. aureus transits from skins/nares to inside the human body, it experiences changes in temperature. The production and content of S. aureus extracellular vesicles (EVs) have been increasingly studied over the past few years, and EVs are increasingly being recognized as important to the infectious process. Nonetheless, the impact of temperature variation on S. aureus EVs has not been studied in detail, as most reports that investigate EV cargoes and host cell interactions are performed using vesicles produced at 37°C. Here, we report that EVs in S. aureus differ in size and protein/RNA cargo depending on the growth temperature used. We demonstrate that the temperature-dependent regulation of vesicle production in S. aureus is mediated by the alpha phenol-soluble modulin peptides (αPSMs). Through proteomic analysis, we observed increased packaging of virulence factors at 40°C, whereas the EV proteome has greater diversity at 34°C. Similar to the protein content, we perform transcriptomic analysis and demonstrate that the RNA cargo also is impacted by temperature. Finally, we demonstrate greater αPSM- and alpha-toxin-mediated erythrocyte lysis with 40°C EVs, but 34°C EVs are more cytotoxic toward THP-1 cells. Together, our study demonstrates that small temperature variations have great impact on EV biogenesis and shape the interaction with host cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.