ArticleThe EMBO journal2021
A dual-function RNA balances carbon uptake and central metabolism in Vibrio cholerae.
Article in The EMBO journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 28 citations in OpenAlex.
- Small regulatory RNAs mediated regulation of virulence and host-pathogen interaction in the Gram-negative ESKAPE pathogens.Virulence · 2026Review
- Bacterial small regulatory RNAs.Nature reviews. Microbiology · 2026Review
- A 3'UTR-derived small RNA modulates the life cycle of the cholera toxin-encoding filamentous phage, CTXϕ.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Phosphate Limitation Modulates Vibrio Cholerae Outer Membrane Vesicle Formation, Composition and Toxicity.Journal of extracellular biology · 2026Article
- YhfH functions as a tri-function RNA in Bacillus thuringiensis BMB171.Nucleic acids research · 2026Article
- From environmental signals to adaptive phenotypes: signal-responsive regulation and network logic of bacterial small RNAs.FEMS microbiology reviews · 2026Review
- Overview of small proteins encoded by bacterial dual-function small RNAs.Non-coding RNA research · 2025Review
- A small RNA fromVirulence · 2025Article
- Review
- Large Roles of Small Proteins.Annual review of microbiology · 2024Review
- Uncovering the small proteome of Methanosarcina mazei using Ribo-seq and peptidomics under different nitrogen conditions.Nature communications · 2024Article
- Insights into bacterial metabolism from small RNAs.Cell chemical biology · 2024Review
- Two for one: regulatory RNAs that encode small proteins.Trends in biochemical sciences · 2023Review
- The RNA-RNA interactome between a phage and its satellite virus reveals a small RNA that differentially regulates gene expression across both genomes.Molecular microbiology · 2023Article
- A Small Non-Coding RNA Mediates Transcript Stability and Expression of Cytochrome bd Ubiquinol Oxidase Subunit I inInternational journal of molecular sciences · 2023Article
- Small regulatory RNAs inmicroLife · 2023Review
- An RNA sponge controls quorum sensing dynamics and biofilm formation in Vibrio cholerae.Nature communications · 2022Article
- Targeting Virulence Genes Expression inInternational journal of molecular sciences · 2022Article
- Glutamine synthetase mRNA releases sRNA from its 3'UTR to regulate carbon/nitrogen metabolic balance ineLife · 2022Article
- Dual-function AzuCR RNA modulates carbon metabolism.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
Bacterial small RNAs (sRNAs) are well known to modulate gene expression by base pairing with trans-encoded transcripts and are typically non-coding. However, several sRNAs have been reported to also contain an open reading frame and thus are considered dual-function RNAs. In this study, we discovered a dual-function RNA from Vibrio cholerae, called VcdRP, harboring a 29 amino acid small protein (VcdP), as well as a base-pairing sequence. Using a forward genetic screen, we identified VcdRP as a repressor of cholera toxin production and link this phenotype to the inhibition of carbon transport by the base-pairing segment of the regulator. By contrast, we demonstrate that the VcdP small protein acts downstream of carbon transport by binding to citrate synthase (GltA), the first enzyme of the citric acid cycle. Interaction of VcdP with GltA results in increased enzyme activity and together VcdR and VcdP reroute carbon metabolism. We further show that transcription of vcdRP is repressed by CRP allowing us to provide a model in which VcdRP employs two different molecular mechanisms to synchronize central metabolism in V. cholerae.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.