Evidence map›Paper›PMID 34611862›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Glycoinformatics Tools for Comprehensive Characterization of Glycans Enzymatically Released from Proteins.

Ian Walsh, Sophie Zhao, Katherine Wongtrakul-Kish, Matthew Choo, Shi Jie Tay, Christopher H Taron, Pauline M Rudd, Terry Nguyen-Khuong

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Ian WalshBioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.
Sophie ZhaoBioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.
Katherine Wongtrakul-KishBioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.
Matthew ChooBioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.
Shi Jie TayBioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.
Christopher H TaronNew England Biolabs, Ipswich, MA, USA.
Pauline M RuddBioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.
Terry Nguyen-KhuongBioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore. terry_nguyen_khuong@bti.a-star.edu.sg.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Glycosylation is important in biology, contributing to both protein conformation and function. Structurally, glycosylation is complex and diverse. This complexity is reflected in the topology, composition, monosaccharide linkages, and isomerism of each oligosaccharide. Glycoanalytics is a discipline that addresses the understanding and characterization of this complexity and its correlation with biology. It includes analytical steps such as sample preparation, instrument measurements, and data analyses. Of these, data analysis has emerged as a critical bottleneck because data collection has increasingly become high-throughput. This has resulted in data-rich workflows that lack rapid and automated data analytics. To address this issue, the field has been developing software for interpretation of quantitative glycomics studies. Here, we describe a protocol using available informatics tools for analysis of data from analysis of released glycans using high-/ultraperformance liquid chromatography (H/UPLC) coupled with mass spectrometry (MS).

Indexed as

GlycomicsChromatography, High Pressure LiquidGlycosylationMass SpectrometryPolysaccharidesPolysaccharidesBioinformaticsGlycoanalyticsGlycosylationMass spectrometry

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.