Evidence map›Paper›PMID 34610337›Full record

ArticleArchives of biochemistry and biophysics2021

Rapid preparation of nanodiscs for biophysical studies.

Jeffrey A Julien, Martin G Fernandez, Katrina M Brandmier, Joshua T Del Mundo, Carol M Bator, Lucie A Loftus, Esther W Gomez, Enrique D Gomez, Kerney Jebrell Glover

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Archives of biochemistry and biophysics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Jeffrey A JulienDepartment of Chemistry, Lehigh University, 6 E. Packer Ave. Bethlehem, Pennsylvania, 18015, USA.
Martin G FernandezDepartment of Chemistry, Lehigh University, 6 E. Packer Ave. Bethlehem, Pennsylvania, 18015, USA.
Katrina M BrandmierDepartment of Chemistry, Lehigh University, 6 E. Packer Ave. Bethlehem, Pennsylvania, 18015, USA.
Joshua T Del MundoDepartment of Chemical Engineering, The Pennsylvania State University, 121 Chemical and Biomedical Engineering Building, University Park, PA, 16802, USA.
Carol M BatorHuck Institutes of Life Sciences, Cryo-EM Facility, The Pennsylvania State University, University Park, PA, 16802, USA.
Lucie A LoftusDepartment of Chemistry, Lehigh University, 6 E. Packer Ave. Bethlehem, Pennsylvania, 18015, USA.
Esther W GomezDepartment of Chemical Engineering, The Pennsylvania State University, 121 Chemical and Biomedical Engineering Building, University Park, PA, 16802, USA.
Enrique D GomezDepartment of Chemical Engineering, The Pennsylvania State University, 121 Chemical and Biomedical Engineering Building, University Park, PA, 16802, USA; Department of Materials Science and Engineering, The Pennsylvania State University, 404 Steidle Building, University Park, PA, 16802, USA.
Kerney Jebrell GloverDepartment of Chemistry, Lehigh University, 6 E. Packer Ave. Bethlehem, Pennsylvania, 18015, USA. Electronic address: kjg206@lehigh.edu.

Funding

Biophysical Studies of CaveolinR15GM141606 · NIGMS · LEHIGH UNIVERSITY · PI GLOVER, KERNEY JEBRELL · 2021 to 2022
$545k
NIGMS NIH HHS R15 GM141606
6 · The paper itself

Abstract

Nanodiscs, which are disc-shaped entities that contain a central lipid bilayer encased by an annulus of amphipathic helices, have emerged as a leading native-like membrane mimic. The current approach for the formation of nanodiscs involves the creation of a mixed-micellar solution containing membrane scaffold protein, lipid, and detergent followed by a time consuming process (3-12 h) of dialysis and/or incubation with sorptive beads to remove the detergent molecules from the sample. In contrast, the methodology described herein provides a facile and rapid procedure for the preparation of nanodiscs in a matter of minutes (<15 min) using Sephadex® G-25 resin to remove the detergent from the sample. A panoply of biophysical techniques including analytical ultracentrifugation, dynamic light scattering, gel filtration chromatography, circular dichroism spectroscopy, and cryogenic electron microscopy were employed to unequivocally confirm that aggregates formed by this method are indeed nanodiscs. We believe that this method will be attractive for time-sensitive and high-throughput experiments.

Indexed as

BiophysicsDimyristoylphosphatidylcholineLipid BilayersMembrane ProteinsMolecular WeightNanostructuresParticle SizeProtein Conformation, alpha-HelicalDimyristoylphosphatidylcholineLipid BilayersMembrane ProteinsAnalytical ultracentrifugationCircular dichroism spectroscopyCryogenic electron microscopyDynamic light scatteringNanodiscsReconstitution

Identifiers

PMID34610337
PMCPMC8596956

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.