Evidence map›Paper›PMID 34609793›Full record

ArticleTransboundary and emerging diseases2022

SARS-CoV-2 RNA stability in dry swabs for longer storage and transport at different temperatures.

Alonzo Alfaro-Núñez, Stephanie Crone, Shila Mortensen, Maiken Worsøe Rosenstierne, Anders Fomsgaard, Ellinor Marving, Sofie Holdflod Nielsen, Michelle Grace Pinto Jørgensen, Charlotta Polacek, Arieh S Cohen and 1 more

Abstract read
In one paragraph

Article in Transboundary and emerging diseases, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Alonzo Alfaro-NúñezDepartment of Virus and Microbiological Special Diagnostics, Statens Serum Institut, Copenhagen S, Denmark.ORCID https://orcid.org/0000-0002-4050-5041
Stephanie CroneSSI Diagnostica, Hillerød, Denmark.
Shila MortensenDepartment of Virus and Microbiological Special Diagnostics, Statens Serum Institut, Copenhagen S, Denmark.
Maiken Worsøe RosenstierneDepartment of Virus and Microbiological Special Diagnostics, Statens Serum Institut, Copenhagen S, Denmark.
Anders FomsgaardDepartment of Virus and Microbiological Special Diagnostics, Statens Serum Institut, Copenhagen S, Denmark.
Ellinor MarvingDepartment of Virus and Microbiological Special Diagnostics, Statens Serum Institut, Copenhagen S, Denmark.
Sofie Holdflod NielsenTestCenter Danmark, Statens Serum Institut, Copenhagen S, Denmark.
Michelle Grace Pinto JørgensenTestCenter Danmark, Statens Serum Institut, Copenhagen S, Denmark.
Charlotta PolacekDepartment of Virus and Microbiological Special Diagnostics, Statens Serum Institut, Copenhagen S, Denmark.
Arieh S CohenTestCenter Danmark, Statens Serum Institut, Copenhagen S, Denmark.
Claus NielsenDepartment of Virus and Microbiological Special Diagnostics, Statens Serum Institut, Copenhagen S, Denmark.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

During the current COVID-19 pandemic, different methods have been used to evaluate patients with suspected SARS-CoV-2 infection. In this study, we experimentally evaluate the ability of spiked saliva-moist swabs and spiked swabs without any transport medium to retain SARS-CoV-2 for storage and transport at different environmental settings during different incubation time periods. Our results show that at ambient temperature of 20°C, SARS-CoV-2 RNA remains stable for up to 9 days allowing a long-time span for transport and storage without compromising clinical results. Additionally, this study demonstrates that saliva-moist swabs can also be stored at -20°C and +4°C for up to 26 days without affecting RT-qPCR results. Our data are relevant for low-and middle-income countries, which have limited access to rapid refrigerated transport and storage of samples representing an economical alternative. Finally, our study demonstrates the practical and economic advantage of using swabs without transport medium.

Indexed as

COVID-19SARS-CoV-2AnimalsPandemicsRNA StabilityRNA, ViralSalivaSpecimen HandlingTemperatureRNA, Viralclinical samplesCOVID-19diagnosticsoropharyngealRNA quantification methodssalivasurveillancetransport mediumvirus stability

Identifiers

PMID34609793
PMCPMC8662091

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.