ArticleJournal of assisted reproduction and genetics2022
Desiccated cat spermatozoa retain DNA integrity and developmental potential after prolonged storage and shipping at non-cryogenic temperatures.
Article in Journal of assisted reproduction and genetics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- One Embryology: impactful connections between human- and wildlife-assisted reproduction.Journal of assisted reproduction and genetics · 2026Review
- Drying parameters and aging modulate protective properties of vitrified trehalose.bioRxiv : the preprint server for biology · 2026Article
- Drying parameters and aging modulate protective properties of vitrified trehalose.BBA advances · 2026Article
- Microwave-Assisted Dehydration, Non-Cryogenic Storage, and Rehydration of Spermatozoa and Germinal Vesicles.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Dehydration-based preservation of cat cumulus-oocyte complexes is not improved by membrane-permeable trehalose.Frontiers in veterinary science · 2026Article
- Reproductive Sciences to the Rescue of Wild Animal Species.Advances in experimental medicine and biology · 2026Review
- Trehalose delivered by cold-responsive nanoparticles improves tolerance of cumulus-oocyte complexes to microwave drying.Journal of assisted reproduction and genetics · 2023Article
- Current State of In Vitro Embryo Production in African Lion (Animals : an open access journal from MDPI · 2022Article
- Long-term preservation of germ cells and gonadal tissues at ambient temperatures.Reproduction & fertility · 2022Review
- Long-term storage of gametes and gonadal tissues at room temperatures: the end of the ice age?Journal of assisted reproduction and genetics · 2022Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo evaluate the DNA integrity and developmental potential of microwave-dehydrated cat spermatozoa after storage at - 20 °C for different time periods and/or overnight shipping on dry ice.
methodsEpididymal spermatozoa from domestic cats were microwave-dehydrated on coverslips after trehalose exposure. Dried samples were either assessed immediately, stored for various duration at - 20 °C, or shipped internationally on dry ice before continued storage. Dry-stored spermatozoa were rehydrated before assessing DNA integrity (TUNEL assays) or developmental potential (injection into in vitro matured oocytes followed by in vitro embryo culture for up to 7 days).
resultsPercentages of dried-rehydrated spermatozoa with intact DNA was not significantly affected (P > 0.05) by desiccation and short-term storage (range, 78.9 to 80.0%) but decreased (P < 0.05) with storage over 5 months (range, 71.0 to 75.2%) compared to fresh controls (92.6 ± 2.2%). After oocyte injection with fresh or dried-rehydrated spermatozoa (regardless of storage time), percentages of activation, pronuclear formation, and embryo development were similar (P > 0.05). Importantly, spermatozoa shipped internationally also retained the ability to support embryo development up to the morula stage.
conclusionResults demonstrated the possibility to sustain DNA integrity and developmental potential of spermatozoa by dry-preservation, even after long-term storage and long-distance shipment at non-cryogenic temperatures. While further studies are warranted, present results demonstrate that dry preservation can be a reliable approach for simple and cost-effective sperm biobanking or shipment.
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