ArticlePlant physiology2021
GLABRA2-based selection efficiently enriches Cas9-generated nonchimeric mutants in the T1 generation.
Article in Plant physiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Evaluation of Transgenerational Gene Editing Efficiency and Inheritance of Edits Using a Split Cas9/gRNA Crossing System in Zea mays.Plant biotechnology journal · 2026Article
- Development of an RNA aptamer-assisted CRISPR/Cas9 system for efficiently generating and isolating Cas9-free mutants in plant.PLoS genetics · 2025Article
- SSA-mediated selection marker gene activation enhances relative gene targeting efficiency in plants.Horticulture research · 2025Article
- Double step screening using endogenous marker improves relative gene targeting efficiency in Arabidopsis.Scientific reports · 2024Article
- A dual-function selection system enables positive selection of multigene CRISPR mutants and negative selection ofaBIOTECH · 2024Article
- XYLEM CYSTEINE PEPTIDASE 1 and its inhibitor CYSTATIN 6 regulate pattern-triggered immunity by modulating the stability of the NADPH oxidase RESPIRATORY BURST OXIDASE HOMOLOG D.The Plant cell · 2024Article
- Usefulness of current sgRNA design guidelines and in vitro cleavage assays for plant CRISPR/Cas genome editing: a case targeting the polyphenol oxidase gene family in eggplant (Solanum melongena L.).Transgenic research · 2023Article
- Liposome-Based Carriers for CRISPR Genome Editing.International journal of molecular sciences · 2023Review
- Strategies and Methods for Improving the Efficiency of CRISPR/Cas9 Gene Editing in Plant Molecular Breeding.Plants (Basel, Switzerland) · 2023Review
- Plant breeding advancements with "CRISPR-Cas" genome editing technologies will assist future food security.Frontiers in plant science · 2023Review
- Arabidopsis Cys2/His2 Zinc Finger Transcription Factor ZAT18 Modulates the Plant Growth-Defense Tradeoff.International journal of molecular sciences · 2022Article
- Application of CRISPR/CasΦ2 System for Genome Editing in Plants.International journal of molecular sciences · 2022Article
- Updates on gene editing and its applications.Plant physiology · 2022Article
- A simple and efficient strategy to produce transgene-free gene edited plants in one generation using paraquat resistant 1 as a selection marker.Frontiers in plant science · 2022Article
- Establishment of an Efficient Genome Editing System in Lettuce Without Sacrificing Specificity.Frontiers in plant science · 2022Article
- Mini-Review: Transgenerational CRISPR/Cas9 Gene Editing in Plants.Frontiers in genome editing · 2022Review
- Transgene-free Genome Editing in Plants.Frontiers in genome editing · 2021Review
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6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The CRISPR/Cas9 system is a widely used tool for genome editing in plants. In Arabidopsis (Arabidopsis thaliana), egg cell-specific promoters driving Cas9 expression have been applied to reduce the proportion of T1 transformants that are chimeras; however, this approach generally leads to relatively low mutagenesis rates. In this study, a GLABRA2 mutation-based visible selection (GBVS) system was established to enrich nonchimeric mutants among T1 plants generated by an egg cell-specific CRISPR/Cas9 system. GBVS generally enhanced mutation screening, increasing the frequency by 2.58- to 7.50-fold, and 25%-48.15% of T1 plants selected through the GBVS system were homozygous or biallelic mutants, which was 1.71- to 7.86-fold higher than the percentage selected using the original system. The mutant phenotypes of T2 plants were not obviously affected by the glabrous background for all four target genes used in this study. Additionally, the nonchimeric pyrabactin resistance 1 (PYR1)/PYR1-like 1 (PYL1) and PYL2 triple mutant pyr1/pyl1/pyl2 could be obtained in the T1 generation with a ratio of 26.67% when GBVS was applied. Collectively, our results show that compared with the known CRISPR/Cas9 systems, the GBVS system described here saves more time and labor when used for the obtainment of homozygous or biallelic monogenic mutants and nonchimeric polygenic mutants in Arabidopsis.
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