ArticlePlant physiology2021
Sheet-like clay nanoparticles deliver RNA into developing pollen to efficiently silence a target gene.
Article in Plant physiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 77 citations in OpenAlex.
- Advancing the adoption of RNA interference-based biopesticides.Nature plants · 2026Review
- Transgene-free genome editing in plants.aBIOTECH · 2026Review
- Genetic Transformation of Catharanthus roseus with Simplified Nanocarrier-Based Gene Delivery Method Using Green Synthesized Superparamagnetic Iron Oxide Nanoparticles.Molecular biotechnology · 2026Article
- Nanoparticle-Mediated Nucleic Acid Delivery Systems in Plant Biotechnology: Recent Advances and Emerging Challenges.Plants (Basel, Switzerland) · 2025Review
- Therapeutic Oligonucleotides for Neurodegenerative Diseases: Aptamer Strategies and Clay Nanoparticle-Based Delivery.Chemical record (New York, N.Y.) · 2025Review
- Application of graphene oxide in Agrobacterium-mediated genetic transformation and construction of a novel DNA delivery system for watermelon.Scientific reports · 2025Article
- Nanodelivery of nucleic acids for plant genetic engineering.Discover nano · 2025Review
- Form and Function: The Factors That Influence the Efficacy of Nanomaterials for Gene Transfer to Plants.Molecules (Basel, Switzerland) · 2025Review
- Lysozyme-coated nanoparticles for active uptake and delivery of synthetic RNA and plasmid-encoded genes in plants.Nature plants · 2025Article
- Nanoparticle-mediated dsRNA delivery for precision insect pest control: a comprehensive review.Molecular biology reports · 2024Review
- Leveraging RNA interference technology for selective and sustainable crop protection.Frontiers in plant science · 2024Review
- Improving RNA-based crop protection through nanotechnology and insights from cross-kingdom RNA trafficking.Current opinion in plant biology · 2023Review
- Nanoplatforms for the Delivery of Nucleic Acids into Plant Cells.International journal of molecular sciences · 2023Review
- RNA-Based Control of Fungal Pathogens in Plants.International journal of molecular sciences · 2023Review
- Nanotechnology in Food and Plant Science: Challenges and Future Prospects.Plants (Basel, Switzerland) · 2023Review
- Technological Development and Application of Plant Genetic Transformation.International journal of molecular sciences · 2023Review
- A vector-free gene interference system using delaminated Mg-Al-lactate layered double hydroxide nanosheets as molecular carriers to intact plant cells.Plant methods · 2023Article
- Recent Progress on Nanocarriers for Topical-Mediated RNAi Strategies for Crop Protection-A Review.Molecules (Basel, Switzerland) · 2023Review
- Strategies for delivery of CRISPR/Cas-mediated genome editing to obtain edited plants directly without transgene integration.Frontiers in genome editing · 2023Review
- Clay nanoparticles efficiently deliver small interfering RNA to intact plant leaf cells.Plant physiology · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Topical application of double-stranded RNA (dsRNA) can induce RNA interference (RNAi) and modify traits in plants without genetic modification. However, delivering dsRNA into plant cells remains challenging. Using developing tomato (Solanum lycopersicum) pollen as a model plant cell system, we demonstrate that layered double hydroxide (LDH) nanoparticles up to 50 nm in diameter are readily internalized, particularly by early bicellular pollen, in both energy-dependent and energy-independent manners and without physical or chemical aids. More importantly, these LDH nanoparticles efficiently deliver dsRNA into tomato pollen within 2-4 h of incubation, resulting in an 89% decrease in transgene reporter mRNA levels in early bicellular pollen 3-d post-treatment, compared with a 37% decrease induced by the same dose of naked dsRNA. The target gene silencing is dependent on the LDH particle size, the dsRNA dose, the LDH-dsRNA complexing ratio, and the treatment time. Our findings indicate that LDH nanoparticles are an effective nonviral vector for the effective delivery of dsRNA and other biomolecules into plant cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.