ArticlePlant physiology2021
Improved virus-induced gene silencing allows discovery of a serpentine synthase gene in Catharanthus roseus.
Article in Plant physiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed, 41 citations in OpenAlex.
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- Phytomelatonin receptor PMTR1 is crucial for melatonin-mediated plant broad-spectrum disease resistance.Plant cell reports · 2026Article
- A cell-type-specific regulon controlling monoterpene indole alkaloid biosynthesis with feedback and feedforward activation loops.The New phytologist · 2026Article
- Red light and biostimulants containing arbuscular mycorrhizal fungi enhance ajmalicine and serpentine production in Catharanthus roseus under indoor conditions.Scientific reports · 2025Article
- Evolution of CYP71D drives the diversification of monoterpene indole alkaloid biosynthesis in Gentianales.Plant communications · 2025Article
- Agrobacterium-mediated transformation for virus-induced gene silencing (VIGS) in Castilleja tenuiflora: Cte-chlH and Cte-PDS.Plant cell reports · 2025Article
- Phylogenomics and metabolic engineering reveal a conserved gene cluster in Solanaceae plants for withanolide biosynthesis.Nature communications · 2025Article
- Oxidative Rearrangements of the Alkaloid Intermediate Geissoschizine.Angewandte Chemie (International ed. in English) · 2025Article
- Establishment of efficient Trichosanthes mottle mosaic virus-derived gene silencing in cucurbit plants.Stress biology · 2025Article
- Navigating Amaryllidaceae alkaloids: bridging gaps and charting biosynthetic territories.Journal of experimental botany · 2025Review
- Cell-type-aware regulatory landscapes governing monoterpene indole alkaloid biosynthesis in the medicinal plant Catharanthus roseus.The New phytologist · 2025Article
- Critical parameters for robustPlant direct · 2024Article
- A Cotyledon-based Virus-Induced Gene Silencing (Cotyledon-VIGS) approach to study specialized metabolism in medicinal plants.Plant methods · 2024Article
- Engineered biosynthesis of plant heteroyohimbine and corynantheine alkaloids in Saccharomyces cerevisiae.Journal of industrial microbiology & biotechnology · 2024Article
- Biosynthesis of natural and halogenated plant monoterpene indole alkaloids in yeast.Nature chemical biology · 2023Article
- Single-cell multi-omics in the medicinal plant Catharanthus roseus.Nature chemical biology · 2023Article
- Safflower Flavonoid 3'5'Hydroxylase Promotes Methyl Jasmonate-Induced Anthocyanin Accumulation in Transgenic Plants.Molecules (Basel, Switzerland) · 2023Article
- Virus-induced gene silencing for in planta validation of gene function in cucurbits.Plant physiology · 2022Article
- Deciphering and reprogramming the cyclization regioselectivity in bifurcation of indole alkaloid biosynthesis.Chemical science · 2022Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Specialized metabolites are chemically complex small molecules with a myriad of biological functions. To investigate plant-specialized metabolite biosynthesis more effectively, we developed an improved method for virus-induced gene silencing (VIGS). We designed a plasmid that incorporates fragments of both the target gene and knockdown marker gene (phytoene desaturase, PDS), which identifies tissues that have been successfully silenced in planta. To demonstrate the utility of this method, we used the terpenoid indole alkaloid (TIA) pathway in Madagascar periwinkle (Catharanthus roseus) as a model system. Catharanthus roseus is a medicinal plant well known for producing many bioactive compounds, such as vinblastine and vincristine. Our VIGS method enabled the discovery of a previously unknown biosynthetic enzyme, serpentine synthase (SS). This enzyme is a cytochrome P450 (CYP) that produces the β-carboline alkaloids serpentine and alstonine, compounds with strong blue autofluorescence and potential pharmacological activity. The discovery of this enzyme highlights the complexity of TIA biosynthesis and demonstrates the utility of this improved VIGS method for discovering unidentified metabolic enzymes in plants.
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