ArticlePlant physiology2021
Simultaneous imaging of ER and cytosolic Ca2+ dynamics reveals long-distance ER Ca2+ waves in plants.
Article in Plant physiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 49 citations in OpenAlex.
- Synthetic ion channel inhibitors enhance plant drought tolerance.Nature communications · 2026Article
- Calcium connections: endoplasmic reticulum CaThe New phytologist · 2026Article
- MAcro Plant Projection Imaging (MAPPI): An open, scalable platform for whole-plant fluorescence real-time imaging.Science advances · 2026Article
- Review
- Inter-Organellar CaCells · 2025Review
- A molecular systems perspective on calcium oscillations beyond ion fluxes.Current opinion in cell biology · 2025Review
- The photosensitive endoplasmic reticulum-chloroplast contact site.Journal of microscopy · 2025Article
- Transformation of flg22 perception into electrical signals decoded in vasculature leads to sieve tube blockage and pathogen resistance.Science advances · 2025Article
- Cellular calcium homeostasis and regulation of its dynamic perturbation.Quantitative plant biology · 2025Review
- Potassium extrusion by plant cells: evolution from an emergency valve to a driver of long-distance transport.The New phytologist · 2025Review
- Straining the root on and off triggers local calcium signalling.Proceedings. Biological sciences · 2023Article
- Calcium imaging: a technique to monitor calcium dynamics in biological systems.Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Review
- Label-free optical interferometric microscopy to characterize morphodynamics in living plants.Frontiers in plant science · 2023Review
- MCU proteins dominate in vivo mitochondrial Ca2+ uptake in Arabidopsis roots.The Plant cell · 2022Article
- Endoplasmic reticulum oxidoreductin provides resilience against reductive stress and hypoxic conditions by mediating luminal redox dynamics.The Plant cell · 2022Article
- Electrifying rhythms in plant cells.Current opinion in cell biology · 2022Review
- Encoding, transmission, decoding, and specificity of calcium signals in plants.Journal of experimental botany · 2022Review
- Auxin analog-induced Ca2+ signaling is independent of inhibition of endosomal aggregation in Arabidopsis roots.Journal of experimental botany · 2022Article
- CamelliA-based simultaneous imaging of Ca2+ dynamics in subcellular compartments.Plant physiology · 2022Article
- Monitoring calcium handling by the plant endoplasmic reticulum with a low-CaThe Plant journal : for cell and molecular biology · 2022Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Calcium ions (Ca2+) play a key role in cell signaling across organisms. In plants, a plethora of environmental and developmental stimuli induce specific Ca2+ increases in the cytosol as well as in different cellular compartments including the endoplasmic reticulum (ER). The ER represents an intracellular Ca2+ store that actively accumulates Ca2+ taken up from the cytosol. By exploiting state-of-the-art genetically encoded Ca2+ indicators, specifically the ER-GCaMP6-210 and R-GECO1, we report the generation and characterization of an Arabidopsis (Arabidopsis thaliana) line that allows for simultaneous imaging of Ca2+ dynamics in both the ER and cytosol at different spatial scales. By performing analyses in single cells, we precisely quantified (1) the time required by the ER to import Ca2+ from the cytosol into the lumen and (2) the time required to observe a cytosolic Ca2+ increase upon the pharmacological inhibition of the ER-localized P-Type IIA Ca2+-ATPases. Furthermore, live imaging of mature, soil-grown plants revealed the existence of a wounding-induced, long-distance ER Ca2+ wave propagating in injured and systemic rosette leaves. This technology enhances high-resolution analyses of intracellular Ca2+ dynamics at the cellular level and in adult organisms and paves the way to develop new methodologies aimed at defining the contribution of subcellular compartments in Ca2+ homeostasis and signaling.
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