ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2022
Cloning and functional analysis of the novel rice blast resistance gene Pi65 in japonica rice.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 27 citations in OpenAlex.
- Genome-wide prediction of cloned disease resistance homologues across the Oryza genus.The plant genome · 2026Article
- Cloning ofPlants (Basel, Switzerland) · 2026Article
- Research Progress on Rice-Blast-Resistance-Related Genes.Plants (Basel, Switzerland) · 2025Review
- A century of advances in molecular genetics and breeding for sustainable resistance to rice blast disease.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Review
- Status on Genetic Resistance to Rice Blast Disease in the Post-Genomic Era.Plants (Basel, Switzerland) · 2025Review
- Protein kinase Pi65 regulates rice blast resistance through phosphorylation-dependent signaling and metabolic reprogramming.Frontiers in genetics · 2025Article
- Molecular breeding approaches for sustainable rice blast management: recent advances and challenges.Frontiers in plant science · 2025Review
- Use of CRISPR Technology in Gene Editing for Tolerance to Biotic Factors in Plants: A Systematic Review.Current issues in molecular biology · 2024Review
- Genome-wide association analysis uncovers rice blast resistance alleles of Ptr and Pia.Communications biology · 2024Article
- Phenazine biosynthesis protein MoPhzF regulates appressorium formation and host infection through canonical metabolic and noncanonical signaling function in Magnaporthe oryzae.The New phytologist · 2024Article
- An update on evolutionary, structural, and functional studies of receptor-like kinases in plants.Frontiers in plant science · 2024Review
- Recent Advances in Effector Research ofBiomolecules · 2023Review
- Approaches to Reduce Rice Blast Disease Using Knowledge from Host Resistance and Pathogen Pathogenicity.International journal of molecular sciences · 2023Review
- Advances in Receptor-like Protein Kinases in Balancing Plant Growth and Stress Responses.Plants (Basel, Switzerland) · 2023Review
- Integrative transcriptomic, proteomic, and phosphoproteomic analysis on the defense response toFrontiers in plant science · 2023Article
- A lineage-specific Exo70 is required for receptor kinase-mediated immunity in barley.Science advances · 2022Article
- Understanding the Dynamics of Blast Resistance in Rice-Journal of fungi (Basel, Switzerland) · 2022Review
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Authors and funding
11 authors at 5 institutions in 2 countries.
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Abstract
key messagePi65, a leucine-rich repeat receptor-like kinase (LRR-RLK) domain cloned from Oryza sativa japonica, is a novel rice blast disease resistance gene. Rice blast seriously threatens rice production worldwide. Utilizing the rice blast resistance gene to breed rice blast-resistant varieties is one of the best ways to control rice blast disease. Using a map-based cloning strategy, we cloned a novel rice blast resistance gene, Pi65, from the resistant variety GangYu129 (abbreviated GY129, Oryza sativa japonica). Overexpression of Pi65 in the susceptible variety LiaoXing1 (abbreviated LX1, Oryza sativa japonica) enhanced rice blast resistance, while knockout of Pi65 in GY129 resulted in susceptibility to rice blast disease. Pi65 encodes two transmembrane domains, with 15 LRR domains and one serine/threonine protein kinase catalytic domain, conferring resistance to isolates of Magnaporthe oryzae (abbreviated M. oryzae) collected from Northeast China. There were sixteen amino acid differences between the Pi65 resistance and susceptible alleles. Compared with the Pi65-resistant allele, the susceptible allele exhibited one LRR domain deletion. Pi65 was constitutively expressed in whole plants, and it could be induced in the early stage of M. oryzae infection. Transcriptome analysis revealed that numerous genes associated with disease resistance were specifically upregulated in GY129 24 h post inoculation (HPI); in contrast, photosynthesis and carbohydrate metabolism-related genes were particularly downregulated at 24 HPI, demonstrating that disease resistance-associated genes were activated in GY129 (carrying Pi65) after rice blast fungal infection and that cellular basal metabolism and energy metabolism were inhibited simultaneously. Our study provides genetic resources for improving rice blast resistance and enriches the study of rice blast resistance mechanisms.
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