Evidence map›Paper›PMID 34608293›Full record

ArticleNature chemical biology2021

Dynamic crotonylation of EB1 by TIP60 ensures accurate spindle positioning in mitosis.

Xiaoyu Song, Fengrui Yang, Xu Liu, Peng Xia, Wu Yin, Zhikai Wang, Yong Wang, Xiao Yuan, Zhen Dou, Kai Jiang and 15 more

Abstract read
PubMed Publisher
In one paragraph

Article in Nature chemical biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.

0numbers the graph read from it
0cells of the map it votes in
49citing papers in PubMed
6.1field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

49 citing papers in PubMed, 72 citations in OpenAlex.

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  7. Akt-elicited phosphorylation of Acapin steers cell migration.Journal of molecular cell biology · 2025
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

25 authors at 8 institutions in 2 countries.

Xiaoyu Song *MOE Key Laboratory for Cellular Dynamics and Hefei National Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, China.ORCID http://orcid.org/0000-0002-7598-0459
Fengrui Yang *MOE Key Laboratory for Cellular Dynamics and Hefei National Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, China.
Xu Liu *MOE Key Laboratory for Cellular Dynamics and Hefei National Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, China.
Peng Xia *MOE Key Laboratory for Cellular Dynamics and Hefei National Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, China.ORCID http://orcid.org/0000-0002-5419-7756
Wu YinMOE Key Laboratory for Cellular Dynamics and Hefei National Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, China.
Zhikai WangMOE Key Laboratory for Cellular Dynamics and Hefei National Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, China.
Yong WangFrontier Science Center for Immunology and Metabolism, Medical Research Institute, Wuhan University, Wuhan, China.
Xiao YuanMOE Key Laboratory for Cellular Dynamics and Hefei National Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, China.
Zhen DouMOE Key Laboratory for Cellular Dynamics and Hefei National Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, China.
Kai JiangFrontier Science Center for Immunology and Metabolism, Medical Research Institute, Wuhan University, Wuhan, China.
Mingming MaMOE Key Laboratory for Cellular Dynamics and Hefei National Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, China.
Bing HuMOE Key Laboratory for Cellular Dynamics and Hefei National Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, China.
Rui ZhangDepartment of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO, USA.ORCID http://orcid.org/0000-0003-3159-9565
Chao XuMOE Key Laboratory for Cellular Dynamics and Hefei National Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, China.ORCID http://orcid.org/0000-0003-0444-7080
Zhiyong ZhangMOE Key Laboratory for Cellular Dynamics and Hefei National Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, China.ORCID http://orcid.org/0000-0001-8202-9672
Ke RuanMOE Key Laboratory for Cellular Dynamics and Hefei National Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, China.ORCID http://orcid.org/0000-0001-9358-0451
Ruijun TianDepartment of Chemistry, Southern University of Science and Technology, Shenzhen, China.ORCID http://orcid.org/0000-0001-9521-5124
Lin LiCenter for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China.ORCID http://orcid.org/0000-0002-3821-7147
Tao LiuState Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing, China.ORCID http://orcid.org/0000-0001-5347-5892
Donald L HillComprehensive Cancer Center, University of Alabama, Birmingham, AL, USA.
Jianye ZangMOE Key Laboratory for Cellular Dynamics and Hefei National Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, China. zangjy@ustc.edu.cn.ORCID http://orcid.org/0000-0003-2648-9783
Xing LiuMOE Key Laboratory for Cellular Dynamics and Hefei National Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, China. xing1017@ustc.edu.cn.ORCID http://orcid.org/0000-0002-7480-5085
Jinsong LiCenter for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China. jsli@sibcb.ac.cn.
Jinke ChengDepartment of Biochemistry, Shanghai Jiaotong University School of Medicine, Shanghai, China. jkcheng@shsmu.edu.cn.ORCID http://orcid.org/0000-0002-4344-5363
Xuebiao YaoMOE Key Laboratory for Cellular Dynamics and Hefei National Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, China. yaoxb@ustc.edu.cn.ORCID http://orcid.org/0000-0001-8982-5911
University of Science and Technology of China · CNChinese Academy of Sciences · CNWuhan University · CNPeking University · CNShanghai Jiao Tong University · CNSouthern University of Science and Technology · CNUniversity of Alabama at Birmingham · USWashington University in St. Louis · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Spindle position control is essential for cell fate determination and organogenesis. Early studies indicate the essential role of the evolutionarily conserved Gαi/LGN/NuMA network in spindle positioning. However, the regulatory mechanisms that couple astral microtubules dynamics to the spindle orientation remain elusive. Here we delineated a new mitosis-specific crotonylation-regulated astral microtubule-EB1-NuMA interaction in mitosis. EB1 is a substrate of TIP60, and TIP60-dependent crotonylation of EB1 tunes accurate spindle positioning in mitosis. Mechanistically, TIP60 crotonylation of EB1 at Lys66 forms a dynamic link between accurate attachment of astral microtubules to the lateral cell cortex defined by NuMA-LGN and fine tune of spindle positioning. Real-time imaging of chromosome movements in HeLa cells expressing genetically encoded crotonylated EB1 revealed the importance of crotonylation dynamics for accurate control of spindle orientation during metaphase-anaphase transition. These findings delineate a general signaling cascade that integrates protein crotonylation with accurate spindle positioning for chromosome stability in mitosis.

Indexed as

Amino Acid SequenceCell Cycle ProteinsChromosomesEscherichia coliHeLa CellsHumansKineticsLysine Acetyltransferase 5Microtubule-Associated ProteinsMicrotubulesMitosisProtein BindingProtein ConformationSpindle ApparatusCell Cycle ProteinsKAT5 protein, humanLysine Acetyltransferase 5MAPRE1 protein, humanMicrotubule-Associated ProteinsNUMA1 protein, human

Identifiers

PMID34608293
OpenAlexW3203065709

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.