ArticleHorticulture research2021
PbrRALF2-elicited reactive oxygen species signaling is mediated by the PbrCrRLK1L13-PbrMPK18 module in pear pollen tubes.
Article in Horticulture research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- Plant Peptides on the Rise: From Historical Insight to Future Applications.Plant biotechnology journal · 2026Review
- Two pear phytocytokines PbePep4 and PdrPep6 interfamilially elicit broad-spectrum immunity against various pathogens in crops.Horticulture research · 2026Article
- Endophytic extract Zhinengcong alleviates salt stress-induced reproductive defects in Solanum lycopersicum.BMC plant biology · 2026Article
- PbrRALF5/10 prevents incompatible pollen tube death by reconstructing the methyl-esterified pectin and reactive oxygen species metabolism of pear in vitro.Plant molecular biology · 2025Article
- The RALF-FERONIA Signaling Axis: A Central Hub Integrating Plant Growth, Reproduction, and Stress Responses.International journal of molecular sciences · 2025Review
- Genome-Wide Characterization ofInternational journal of molecular sciences · 2025Article
- Investigating vesicle-mediated regulation of pollen tube growth through BFA inhibition and AS-ODN targeting ofHorticulture research · 2025Article
- PbrBGAL6 promotes pollen tube growth by influencing apical pectin level in Pyrus bretschneideri.BMC genomics · 2025Article
- Peptide hormones in plants.Molecular horticulture · 2025Review
- Self S-RNase reduces the expression of two pollen-specific COBRA genes to inhibit pollen tube growth in pear.Molecular horticulture · 2023Article
- Characterization of Malectin/Malectin-like Receptor-like Kinase Family Members in Foxtail Millet (Life (Basel, Switzerland) · 2023Article
- Rapid alkalinization factor: function, regulation, and potential applications in agriculture.Stress biology · 2023Review
- Signals and Their Perception for Remodelling, Adjustment and Repair of the Plant Cell Wall.International journal of molecular sciences · 2023Review
- PbrCalS5, a callose synthase protein, is involved in pollen tube growth in Pyrus bretschneideri.Planta · 2022Article
- Endophytic extract Zhinengcong alleviates heat stress-induced reproductive defect inFrontiers in plant science · 2022Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rapid alkalinization factors (RALFs) are cysteine-rich peptides that play important roles in a variety of biological processes, such as cell elongation and immune signaling. Recent studies in Arabidopsis have shown that RALFs regulate pollen tube growth via plasma membrane receptor-like kinases (RLKs). However, the downstream signal transduction mechanisms of RLKs in pollen tubes are unknown. Here, we identified PbrRALF2, a pear (Pyrus bretschneideri) pollen RALF peptide that inhibits pollen tube growth. We found that PbrRALF2 interacts with a malectin-like domain-containing RLK, PbrCrRLK1L13. The relative affinity between PbrRALF2 and PbrCrRLK1L13 was at the submicromolar level, which is consistent with the values of ligand-receptor kinase pairs and the physiological concentration for PbrRALF2-mediated inhibition of pollen tube growth. After binding to its extracellular domain, PbrRALF2 activated the phosphorylation of PbrCrRLK1L13 in a dose-dependent manner. We further showed that the MAP kinase PbrMPK18 is a downstream target of PbrCrRLK1L13 that mediates PbrRALF2-elicited reactive oxygen species (ROS) production. The excessive accumulation of ROS inhibits pollen tube growth. We show that MPK acts as a mediator for CrRLK1L to stimulate ROS production, which might represent a general mechanism by which RALF and CrRLK1L function in signaling pathways.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.