ArticleBMC microbiology2021
The effect of ArcA on the growth, motility, biofilm formation, and virulence of Plesiomonas shigelloides.
Article in BMC microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Roles of Response Regulators in the Two-Component System in the Formation of Stress Tolerance, Motility and Biofilm inFoods (Basel, Switzerland) · 2024Article
- The effects of PstR, a PadR family transcriptional regulatory factor, in Plesiomonas shigelloides are revealed by transcriptomics.BMC microbiology · 2024Article
- Transcriptome Analysis Reveals Cross-Talk between the Flagellar Transcriptional Hierarchy and Secretion System inInternational journal of molecular sciences · 2024Article
- The Trimeric Autotransporter Adhesin EmaA and Infective Endocarditis.Pathogens (Basel, Switzerland) · 2024Review
- DegS regulates the aerobic metabolism ofFrontiers in cellular and infection microbiology · 2024Article
- Transcriptome Analysis Reveals the Effect of PdhR inInternational journal of molecular sciences · 2023Article
- Regulation of adhesin synthesis in Aggregatibacter actinomycetemcomitans.Molecular oral microbiology · 2023Article
- RpoN is required for the motility and contributes to the killing ability of Plesiomonas shigelloides.BMC microbiology · 2022Article
- Genotyping and biofilm formation of Mycoplasma hyopneumoniae and their association with virulence.Veterinary research · 2022Article
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7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundThe anoxic redox control binary system plays an important role in the response to oxygen as a signal in the environment. In particular, phosphorylated ArcA, as a global transcription factor, binds to the promoter regions of its target genes to regulate the expression of aerobic and anaerobic metabolism genes. However, the function of ArcA in Plesiomonas shigelloides is unknown.
resultsIn the present study, P. shigelloides was used as the research object. The differences in growth, motility, biofilm formation, and virulence between the WT strain and the ΔarcA isogenic deletion mutant strain were compared. The data showed that the absence of arcA not only caused growth retardation of P. shigelloides in the log phase, but also greatly reduced the glucose utilization in M9 medium before the stationary phase. The motility of the ΔarcA mutant strain was either greatly reduced when grown in swim agar, or basically lost when grown in swarm agar. The electrophoretic mobility shift assay results showed that ArcA bound to the promoter regions of the flaK, rpoN, and cheV genes, indicating that ArcA directly regulates the expression of these three motility-related genes in P. shigelloides. Meanwhile, the ability of the ΔarcA strain to infect Caco-2 cells was reduced by 40%; on the contrary, its biofilm formation was enhanced. Furthermore, the complementation of the WT arcA gene from pBAD33-arcA
conclusionsWe showed the effect of ArcA on the growth, motility, biofilm formation, and virulence of Plesiomonas shigelloides, and demonstrated that ArcA functions as a positive regulator controls the motility of P. shigelloides by directly regulating the expression of flaK, rpoN and cheV genes.
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