Evidence map›Paper›PMID 34607258›Full record

ArticleBiochemical and biophysical research communications2021

Growth and mineralization of osteoblasts from mesenchymal stem cells on microporous membranes: Epithelial-like growth with transmembrane resistance and pH gradient.

Quitterie C Larrouture, Irina L Tourkova, Donna B Stolz, Vladimir Riazanski, Kelechi M Onwuka, Jonathan M Franks, Steven F Dobrowolski, Deborah J Nelson, Paul H Schlesinger, Harry C Blair

Open access · greenAbstract read
In one paragraph

Article in Biochemical and biophysical research communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.7field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 11 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Quitterie C LarroutureDepartment of Pathology, University of Pittsburgh, School of Medicine, Pittsburgh, PA, USA.
Irina L TourkovaDepartment of Pathology, University of Pittsburgh, School of Medicine, Pittsburgh, PA, USA; Pittsburgh Veteran's Affairs Medical Center, Pittsburgh, PA, USA.
Donna B StolzDepartment of Cell Biology, University of Pittsburgh, School of Medicine, Pittsburgh, PA, USA.
Vladimir RiazanskiDept of Neurobiology, Pharmacology & Physiology, University of Chicago, Chicago IL, USA.
Kelechi M OnwukaDepartment of Pathology, University of Pittsburgh, School of Medicine, Pittsburgh, PA, USA.
Jonathan M FranksDepartment of Cell Biology, University of Pittsburgh, School of Medicine, Pittsburgh, PA, USA.
Steven F DobrowolskiDepartment of Pathology, University of Pittsburgh, School of Medicine, Pittsburgh, PA, USA.
Deborah J NelsonDept of Neurobiology, Pharmacology & Physiology, University of Chicago, Chicago IL, USA.
Paul H SchlesingerDept of Cell Biology, Washington University, Saint Louis, MO, USA.
Harry C BlairDepartment of Pathology, University of Pittsburgh, School of Medicine, Pittsburgh, PA, USA; Pittsburgh Veteran's Affairs Medical Center, Pittsburgh, PA, USA. Electronic address: hcblair@pitt.edu.
University of Pittsburgh · USUniversity of Chicago · USWashington University in St. Louis · US

Funding

Phagosomal Ion Channels as Therapeutic TargetsR01HL125076 · NHLBI · UNIVERSITY OF CHICAGO · PI NELSON, DEBORAH J. · 2015 to 2022
$5.0M
Epithelial Osteoblast Function: The Role of Acid TransportR01AR076146 · NIAMS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI BLAIR, HARRY COLBERT, NELSON, DEBORAH J. · 2020 to 2024
$2.6M
Epithelial Osteoblast Function: The Role of Acid TransportR56AR076146 · NIAMS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI BLAIR, HARRY COLBERT, NELSON, DEBORAH J. · 2019 to 2019
$652k
Regulation of Osteoblasts by ACTH and VEGFI01BX002490 · VA · VETERANS HEALTH ADMINISTRATION · PI BLAIR, HARRY COLBERT · 2015 to 2025
–
BLRD VA I01 BX002490NHLBI NIH HHS R01 HL125076NIAMS NIH HHS R01 AR076146NIAMS NIH HHS R56 AR076146
6 · The paper itself

Abstract

Osteoblasts in vivo form an epithelial-like layer with tight junctions between cells. Bone formation involves mineral transport into the matrix and acid transport to balance pH levels. To study the importance of the pH gradient in vitro, we used Transwell inserts composed of polyethylene terephthalate (PET) membranes with 0.4 μm pores at a density of (2 ± 0.4) x 10

Indexed as

AnimalsCalcification, PhysiologicCell ProliferationCells, CulturedEpithelial CellsHydrogen-Ion ConcentrationMembranes, ArtificialMesenchymal Stem CellsMiceMice, Inbred C57BLOsteoblastsOsteogenesisPolyethylene TerephthalatesMembranes, ArtificialPolyethylene TerephthalatesAcid transportEpithelial-like osteoblastsMineralizationMSC

Identifiers

PMID34607258
PMCPMC8530971
OpenAlexW3202193496

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.