ArticleFrontiers in cell and developmental biology2021
Transcriptome Analysis Reveals an Inhibitory Effect of Dihydrotestosterone-Treated 2D- and 3D-Cultured Dermal Papilla Cells on Hair Follicle Growth.
Article in Frontiers in cell and developmental biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 23 citations in OpenAlex.
- The central role of fibroblasts in androgenic alopecia: A systematic umbrella review.Cell transplantationPooled it
- Efficacy and safety of topical finasteride spray solution in the treatment of Chinese men with androgenetic alopecia: A phase III, multicenter, randomized, double-blind, placebo-controlled study.Chinese medical journal · 2026Trial
- Systematic standardization of organ-on-a-chip under controlled flow conditions: Use case with Caki-1 and A549 cell lines.MethodsX · 2026Article
- Article
- Bio-inspired suction cup microneedle loaded fusion membrane targeting nano-drug delivery system treat androgenetic alopecia.Journal of nanobiotechnology · 2026Article
- An Insight on Ellagic Acid Formulations for the Management of Skin Diseases.Molecules (Basel, Switzerland) · 2025Review
- CXCL12 Drives Reversible Fibroimmune Remodeling in Androgenetic Alopecia Revealed by Single-Cell RNA Sequencing.International journal of molecular sciences · 2025Article
- Proteomics Reveals the Role of PLIN2 in Regulating the Secondary Hair Follicle Cycle in Cashmere Goats.International journal of molecular sciences · 2025Article
- Immune and Non-immune Interactions in the Pathogenesis of Androgenetic Alopecia.Clinical reviews in allergy & immunology · 2025Review
- Black phosphorus nanosheets encapsulated microneedle for multifunctional therapy for androgenic alopecia.Journal of nanobiotechnology · 2025Article
- Androgenetic Alopecia: An Update on Pathogenesis and Pharmacological Treatment.Drug design, development and therapy · 2025Review
- Jiawei Erzhiwan Ameliorates Androgenetic Alopecia by Regulating the SIRT1/JNK/p38 MAPK Pathway.Drug design, development and therapy · 2025Article
- Subcutaneous injection of genetically engineered exosomes for androgenic alopecia treatment.Frontiers in bioengineering and biotechnology · 2025Article
- Application of multi-omics techniques to androgenetic alopecia: Current status and perspectives.Computational and structural biotechnology journal · 2024Review
- Dendrobium officinale polysaccharide promotes angiogenesis as well as follicle regeneration and hair growth through activation of the WNT signaling pathway.Regenerative therapy · 2024Article
- Adipose-Derived Stem Cell Exosomes Antagonize the Inhibitory Effect of Dihydrotestosterone on Hair Follicle Growth by Activating Wnt/Stem cells international · 2023Article
- Extracellular vesicles from 3D cultured dermal papilla cells improve wound healing via Krüppel-like factor 4/vascular endothelial growth factor A -driven angiogenesis.Burns & trauma · 2023Article
- Limonin, a Component of Immature Citrus Fruits, Activates Anagen Signaling in Dermal Papilla Cells.Nutrients · 2022Article
- Direct Reprograming of Mouse Fibroblasts into Dermal Papilla Cells via Small Molecules.International journal of molecular sciences · 2022Article
- Editorial: Hair Follicle Stem Cell Regeneration in Aging.Frontiers in cell and developmental biology · 2021Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dermal papillae are a target of androgen action in patients with androgenic alopecia, where androgen acts on the epidermis of hair follicles in a paracrine manner. To mimic the complexity of the dermal papilla microenvironment, a better culture model of human dermal papilla cells (DPCs) is needed. Therefore, we evaluated the inhibitory effect of dihydrotestosterone (DHT)-treated two-dimensional (2D)- and 3D-cultured DPCs on hair follicle growth. 2D- and 3D-cultured DPC proliferation was inhibited after co-culturing with outer root sheath (ORS) cells under DHT treatment. Moreover, gene expression levels of β-catenin and neural cell adhesion molecules were significantly decreased and those of cleaved caspase-3 significantly increased in 2D- and 3D-cultured DPCs with increasing DHT concentrations. ORS cell proliferation also significantly increased after co-culturing in the control-3D model compared with the control-2D model. Ki67 downregulation and cleaved caspase-3 upregulation in DHT-treated 2D and 3D groups significantly inhibited ORS cell proliferation. Sequencing showed an increase in the expression of genes related to extracellular matrix synthesis in the 3D model group. Additionally, the top 10 hub genes were identified, and the expression of nine chemokine-related genes in DHT-treated DPCs was found to be significantly increased. We also identified the interactions between transcription factor (TF) genes and microRNAs (miRNAs) with hub genes and the TF-miRNA coregulatory network. Overall, the findings indicate that 3D-cultured DPCs are more representative of
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.