ArticleChemical science2021
Clustering of catalytic nanocompartments for enhancing an extracellular non-native cascade reaction.
Article in Chemical science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed.
- Nature-Inspired Enzymatic Cascades: Emerging Strategies for Sustainable Chemistry.Molecules (Basel, Switzerland) · 2026Review
- Polymeric Giant Unilamellar Vesicles Support Longevity of Native Nuclei in Protocells.Small science · 2025Article
- Design and Applications of Polymersomes for Oral Drug Administration.ACS applied materials & interfaces · 2025Review
- Photoreceptor-Like Signal Transduction Between Polymer-Based Protocells.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Glycooligomer-Functionalized Catalytic Nanocompartments Co-Loaded with Enzymes Support Parallel Reactions and Promote Cell Internalization.Biomacromolecules · 2024Article
- Programmable Enzymatic Reaction Network in Artificial Cell-Like Polymersomes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Tweaking the NRF2 signaling cascade in human myelogenous leukemia cells by artificial nano-organelles.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Microfluidic Giant Polymer Vesicles Equipped with Biopores for High-Throughput Screening of Bacteria.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Lipid vesicle-based molecular robots.Lab on a chip · 2024Review
- Synthetic Cells Revisited: Artificial Cells Construction Using Polymeric Building Blocks.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Enzyme Cascade Electrode Reactions with Nanomaterials and Their Applicability towards Biosensor and Biofuel Cells.Biosensors · 2023Review
- A transient vesicular glue for amplification and temporal regulation of biocatalytic reaction networks.Chemical science · 2023Article
- Current Perspectives on Synthetic Compartments for Biomedical Applications.International journal of molecular sciences · 2022Review
- DNA-directed coimmobilization of multiple enzymes on organic-inorganic hybrid DNA flowers.Frontiers in bioengineering and biotechnology · 2022Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Compartmentalization is fundamental in nature, where the spatial segregation of biochemical reactions within and between cells ensures optimal conditions for the regulation of cascade reactions. While the distance between compartments or their interaction are essential parameters supporting the efficiency of bio-reactions, so far they have not been exploited to regulate cascade reactions between bioinspired catalytic nanocompartments. Here, we generate individual catalytic nanocompartments (CNCs) by encapsulating within polymersomes or attaching to their surface enzymes involved in a cascade reaction and then, tether the polymersomes together into clusters. By conjugating complementary DNA strands to the polymersomes' surface, DNA hybridization drove the clusterization process of enzyme-loaded polymersomes and controlled the distance between the respective catalytic nanocompartments. Owing to the close proximity of CNCs within clusters and the overall stability of the cluster architecture, the cascade reaction between spatially segregated enzymes was significantly more efficient than when the catalytic nanocompartments were not linked together by DNA duplexes. Additionally, residual DNA single strands that were not engaged in clustering, allowed for an interaction of the clusters with the cell surface as evidenced by A549 cells, where clusters decorating the surface endowed the cells with a non-native enzymatic cascade. The self-organization into clusters of catalytic nanocompartments confining different enzymes of a cascade reaction allows for a distance control of the reaction spaces which opens new avenues for highly efficient applications in domains such as catalysis or nanomedicine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.