ArticleFrontiers in microbiology2021
Detection of Bioavailable Cadmium by Double-Color Fluorescence Based on a Dual-Sensing Bioreporter System.
Article in Frontiers in microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 43 citations in OpenAlex.
- Review
- Repurposing the Sb(III)-specific efflux and sequestration system (ant operon) to mitigate antimonite cross-talk in ArsR-based bacterial arsenite sensors.Microbial cell factories · 2025Article
- Multichannel bioelectronic sensing using engineered Escherichia coli.Nature communications · 2025Article
- Heavy Metal-Gut Microbiota Interactions: Probiotics Modulation and Biosensors Detection.Biosensors · 2025Review
- Advancing cadmium bioremediation: future directions for CadR display strategies.Frontiers in bioengineering and biotechnology · 2025Article
- Systematic evaluation of pigment-based whole-cell lead biosensors: challenges in genetic circuit engineering and critical considerations for background noise control.Frontiers in bioengineering and biotechnology · 2025Article
- Detection of environmental pollutants using transcription factor-based whole-cell biosensors.Biotechnology for the environment · 2025Review
- Optimizing chromosome dispersion quality: the key role of cell density.Frontiers in cell and developmental biology · 2025Article
- Improvement of a highly sensitive and specific whole-cell biosensor by adding a positive feedback amplifier.Synthetic and systems biotechnology · 2023Article
- Pb(II)-inducible proviolacein biosynthesis enables a dual-color biosensor toward environmental lead.Frontiers in microbiology · 2023Article
- Metabolic engineering of the carotenoid biosynthetic pathway toward a specific and sensitive inorganic mercury biosensor.RSC advances · 2022Article
- Synthetic biology techniques to tackle heavy metal pollution and poisoning.Synthetic and systems biotechnology · 2022Review
- Fluorescent indicators for live-cell and in vitro detection of inorganic cadmium dynamics.Journal of fluorescence · 2022Article
- Detection of environmental pollutant cadmium in water using a visual bacterial biosensor.Scientific reports · 2022Article
- Anthocyanin biosynthetic pathway switched by metalloregulator PbrR to enable a biosensor for the detection of lead toxicity.Frontiers in microbiology · 2022Article
- Use of whole-cell bioreporters to assess bioavailability of contaminants in aquatic systems.Frontiers in chemistry · 2022Review
- Design of Pb(II)-SpecificFrontiers in microbiology · 2022Article
- Differential Detection of Bioavailable Mercury and Cadmium Based on a Robust Dual-Sensing Bacterial Biosensor.Frontiers in microbiology · 2022Article
- Recent advances in bacterial biosensing and bioremediation of cadmium pollution: a mini-review.World journal of microbiology & biotechnology · 2021Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cadmium (Cd) is carcinogenic to humans and can accumulate in the liver, kidneys, and bones. There is widespread presence of cadmium in the environment as a consequence of anthropogenic activities. It is important to detect cadmium in the environment to prevent further exposure to humans. Previous whole-cell biosensor designs were focused on single-sensing constructs but have had difficulty in distinguishing cadmium from other metal ions such as lead (Pb) and mercury (Hg). We developed a dual-sensing bacterial bioreporter system to detect bioavailable cadmium by employing CadC and CadR as separate metal sensory elements and eGFP and mCherry as fluorescent reporters in one genetic construct. The capability of this dual-sensing biosensor was proved to simultaneously detect bioavailable cadmium and its toxic effects using two sets of sensing systems while still maintaining similar specificity and sensitivity of respective signal-sensing biosensors. The productions of double-color fluorescence were directly proportional to the exposure concentration of cadmium, thereby serving as an effective quantitative biosensor to detect bioavailable cadmium. This novel dual-sensing biosensor was then validated to respond to Cd(II) spiked in environmental water samples. This is the first report of the development of a novel dual-sensing, whole-cell biosensor for simultaneous detection of bioavailable cadmium. The application of two biosensing modules provides versatile biosensing signals and improved performance that can make a significant impact on monitoring high concentration of bioavailable Cd(II) in environmental water to reduce human exposure to the harmful effects of cadmium.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.