ArticlePlant physiology2022
NtMYB305a binds to the jasmonate-responsive GAG region of NtPMT1a promoter to regulate nicotine biosynthesis.
Article in Plant physiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 30 citations in OpenAlex.
- A negative feedback cascade controlling jasmonate-induced nicotine biosynthesis revealed by an in-plate nicotine assay.Molecular horticulture · 2026Article
- Natural variation in OsMYB305 downregulating cytokinin-mediated inhibition of leaf senescence contributes to regional adaptation in rice.The New phytologist · 2026Article
- Metabolic engineering for tobacco quality improvement: Advances and perspectives.Metabolic engineering communications · 2026Review
- Transcriptional Activation of NaNAC72 Suppresses Nicotine Biosynthesis in DNA Methyltransferase NaDRM2-like2 Mutants of Nicotiana attenuata.Plant biotechnology journal · 2026Article
- Genome-wide identification, expression analysis of the JmjC gene family in tobacco and functional characterization of NtJMJ19 role in nicotine biosynthesis.BMC plant biology · 2026Article
- Integrative comparative proteomics identifies core differentially expressed proteins and pathways for drought tolerance by exploiting contrasting rice varieties.Frontiers in plant science · 2026Article
- Comparative analysis of the metabolite compositions and antioxidant capacity of waxy and non-waxy black rice (Food chemistry: X · 2025Article
- SmbZIP5 Integrates Abscisic and Jasmonic Acid Signalling to Positively Regulate Tanshinone Biosynthesis in Salvia miltiorrhiza.Plant biotechnology journal · 2025Article
- Multidimensional regulation of transcription factors: decoding the comprehensive signals of plant secondary metabolism.Frontiers in plant science · 2025Review
- DNA methylation valley as a distinguishing feature occurs in root-specific expressed nicotine-related genes inFrontiers in plant science · 2025Article
- Genetic regulation and manipulation of nicotine biosynthesis in tobacco: strategies to eliminate addictive alkaloids.Journal of experimental botany · 2024Review
- Proteomics and Co-expression Network Analysis Reveal the Importance of Hub Proteins and Metabolic Pathways in Nicotine Synthesis and Accumulation in Tobacco (Frontiers in plant science · 2022Article
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Authors and funding
15 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MYB transcription factors play essential roles in regulating plant secondary metabolism and jasmonate (JA) signaling. Putrescine N-methyltransferase is a key JA-regulated step in the biosynthesis of nicotine, an alkaloidal compound highly accumulated in Nicotiana spp. Here we report the identification of NtMYB305a in tobacco (Nicotiana tabacum) as a regulatory component of nicotine biosynthesis and demonstrate that it binds to the JA-responsive GAG region, which comprises a G-box, an AT-rich motif, and a GCC-box-like element, in the NtPMT1a promoter. Yeast one-hybrid analysis, electrophoretic mobility shift assay and chromatin immunoprecipitation assays showed that NtMYB305a binds to the GAG region in vitro and in vivo. Binding specifically occurs at the ∼30-bp AT-rich motif in a G/C-base-independent manner, thus defining the AT-rich motif as previously unknown MYB-binding element. NtMYB305a localized in the nucleus of tobacco cells where it is capable of activating the expression of a 4×GAG-driven GUS reporter in an AT-rich motif-dependent manner. NtMYB305a positively regulates nicotine biosynthesis and the expression of NtPMT and other nicotine pathway genes. NtMYB305a acts synergistically with NtMYC2a to regulate nicotine biosynthesis, but no interaction between these two proteins was detected. This identification of NtMYB305a provides insights into the regulation of nicotine biosynthesis and extends the roles played by MYB transcription factors in plant secondary metabolism.
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