ArticleCell & bioscience2021
A single-cell interactome of human tooth germ from growing third molar elucidates signaling networks regulating dental development.
Article in Cell & bioscience, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 25 citations in OpenAlex.
- Identification of GLDNInternational journal of oral science · 2026Article
- Resolving tooth development at single-cell resolution: advancing dental regeneration.Frontiers in cell and developmental biology · 2026Review
- Article
- Unveiling the dental cellular landscape: A comprehensive review of single-cell RNA sequencing in human dental tissues.The Japanese dental science review · 2025Review
- Single-cell transcriptomics identifies PDGFRAInternational journal of oral science · 2025Article
- Prediction of hub genes in pulpal inflammation and regeneration using autoencoders and a generative AI approach.Scientific reports · 2025Article
- The paradigm shifts of periodontal regeneration strategy: From reparative manipulation to developmental engineering.Bioactive materials · 2025Review
- The landscape of cell regulatory and communication networks in the human dental follicle.Frontiers in bioengineering and biotechnology · 2025Article
- Decoding dental mesenchymal stem cells diversity: single-cell transcriptomics maps heterogeneity in molar development.Frontiers in cell and developmental biology · 2025Review
- Unraveling the hidden complexity: Exploring dental tissues through single-cell transcriptional profiling.Regenerative therapy · 2024Review
- Single-cell RNA-seq analysis of rat molars reveals cell identity and driver genes associated with dental mesenchymal cell differentiation.BMC biology · 2024Article
- Spatiotemporal cell landscape of human embryonic tooth development.Cell proliferation · 2024Article
- Molecular insights into the proteomic composition of porcine treated dentin matrix.Materials today. Bio · 2024Article
- Effect of Long Non-coding RNA and DNA Methylation on Gene Expression in Dental Fluorosis.Biological trace element research · 2024Article
- Spatiotemporal cellular dynamics and molecular regulation of tooth root ontogeny.International journal of oral science · 2023Review
- FGFR1 variants contributed to families with tooth agenesis.Human genomics · 2023Article
- Single-cell RNA sequencing in oral science: Current awareness and perspectives.Cell proliferation · 2022Review
- Establishment of inclusive single-cell transcriptome atlases from mouse and human tooth as powerful resource for dental research.Frontiers in cell and developmental biology · 2022Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
backgroundDevelopment of dental tissue is regulated by extensive cell crosstalk based on various signaling molecules, such as bone morphogenetic protein (BMP) and fibroblast growth factor (FGF) pathways. However, an intact network of the intercellular regulation is still lacking.
resultTo gain an unbiased and comprehensive view of this dental cell interactome, we applied single-cell RNA-seq on immature human tooth germ of the growing third molar, discovered refined cell subtypes, and applied multiple network analysis to identify the central signaling pathways. We found that immune cells made up over 80% of all tooth germ cells, which exhibited profound regulation on dental cells via Transforming growth factor-β, Tumor necrosis factor (TNF) and Interleukin-1. During osteoblast differentiation, expression of genes related to extracellular matrix and mineralization was continuously elevated by signals from BMP and FGF family. As for the self-renewal of apical papilla stem cell, BMP-FGFR1-MSX1 pathway directly regulated the G0-to-S cell cycle transition. We also confirmed that Colony Stimulating Factor 1 secreted from pericyte and TNF Superfamily Member 11 secreted from osteoblast regulated a large proportion of genes related to osteoclast transformation from macrophage and monocyte.
conclusionsWe constructed the intercellular signaling networks that regulated the essential developmental process of human tooth, which served as a foundation for future dental regeneration engineering and the understanding of oral pathology.
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