Evidence map›Paper›PMID 34600578›Full record

ArticleVeterinary research2021

On-farm colorimetric detection of Pasteurella multocida, Mannheimia haemolytica, and Histophilus somni in crude bovine nasal samples.

Ana Pascual-Garrigos, Murali Kannan Maruthamuthu, Aaron Ault, Josiah Levi Davidson, Grigorii Rudakov, Deepti Pillai, Jennifer Koziol, Jon P Schoonmaker, Timothy Johnson, Mohit S Verma

Open access · goldAbstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Veterinary research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed
4.5field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 47 citations in OpenAlex.

  1. Article
  2. Article
  3. Detection ofAnimals : an open access journal from MDPI · 2026
    Article
  4. Article
  5. Review
  6. CyranoseVeterinary sciences · 2025
    Article
  7. Article
  8. Article
  9. Article
  10. Exploring the diagnostic landscape ofFrontiers in microbiology · 2025
    Review
  11. Review
  12. Article
  13. Understanding theFrontiers in parasitology · 2024
    Review
  14. Review
  15. Article
  16. Article
  17. Review
  18. Review
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Ana Pascual-GarrigosDepartment of Agricultural and Biological Engineering, Purdue University, 225 S University Street, West Lafayette, IN, 47907, USA.
Murali Kannan MaruthamuthuDepartment of Agricultural and Biological Engineering, Purdue University, 225 S University Street, West Lafayette, IN, 47907, USA.
Aaron AultSchool of Electrical and Computer Engineering, Purdue University, 465 Northwestern Avenue, West Lafayette, IN, 47907, USA.
Josiah Levi DavidsonDepartment of Agricultural and Biological Engineering, Purdue University, 225 S University Street, West Lafayette, IN, 47907, USA.
Grigorii RudakovDepartment of Agricultural and Biological Engineering, Purdue University, 225 S University Street, West Lafayette, IN, 47907, USA.
Deepti PillaiDepartment of Comparative Pathobiology, Purdue University, 625 Harrison Street, West Lafayette, IN, 47907, USA.
Jennifer KoziolSchool of Veterinary Medicine, Texas Tech University, 7671 Evans Drive , Amarillo , TX, 79106, USA.
Jon P SchoonmakerDepartment of Animal Sciences, Purdue University, 270 S Russell Street, West Lafayette, IN, 47907, USA.
Timothy JohnsonDepartment of Animal Sciences, Purdue University, 270 S Russell Street, West Lafayette, IN, 47907, USA.
Mohit S VermaDepartment of Agricultural and Biological Engineering, Purdue University, 225 S University Street, West Lafayette, IN, 47907, USA. msverma@purdue.edu.ORCID http://orcid.org/0000-0002-6374-3333
Purdue University West Lafayette · USTexas Tech University · US

Funding

National Institute of Food and Agriculture 2020-68014-31302
6 · The paper itself

Abstract

This work modifies a loop-mediated isothermal amplification (LAMP) assay to detect the bovine respiratory disease (BRD) bacterial pathogens Pasteurella multocida, Mannheimia haemolytica, and Histophilus somni in a colorimetric format on a farm. BRD causes a significant health and economic burden worldwide that partially stems from the challenges involved in determining the pathogens causing the disease. Methods such as polymerase chain reaction (PCR) have the potential to identify the causative pathogens but require lab equipment and extensive sample processing making the process lengthy and expensive. To combat this limitation, LAMP allows accurate pathogen detection in unprocessed samples by the naked eye allowing for potentially faster and more precise diagnostics on the farm. The assay developed here offers 66.7-100% analytical sensitivity, and 100% analytical specificity (using contrived samples) while providing 60-100% concordance with PCR results when tested on five steers in a feedlot. The use of a consumer-grade water bath enabled on-farm execution by collecting a nasal swab from cattle and provided a colorimetric result within 60 min. Such an assay holds the potential to provide rapid pen-side diagnostics to cattle producers and veterinarians.

Indexed as

AnimalsCattleCattle DiseasesColorimetryDiagnostic Tests, RoutineMannheimia haemolyticaMolecular Diagnostic TechniquesNoseNucleic Acid Amplification TechniquesPasteurellaceaePasteurellaceae InfectionsPasteurella InfectionsPasteurella multocidabovine respiratory diseasediagnosticsloop-mediated isothermal amplificationpen-side

Identifiers

PMID34600578
PMCPMC8487530
OpenAlexW3202701627

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.